Recombinant Mouse Antibody (79D) is capable of binding to Kit. The inhibitory KIT antibodies that bind to the membrane-proximal Ig-like D4 of KIT with significant overlap with an epitope in D4 that mediates homotypic interactions essential for KIT activation.
Figure 1 WT KIT activation is inhibited by anti-D4 antibodies.
NIH 3T3 cells expressing WT KIT were incubated for 5 h with the indicated concentrations (Upper) of Fab79D following stimulation with 25 ng/mL of SCF for 5 minat 37 °C. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation (IP) with anti-KIT antibodies followed by SDS/PAGE and immunoblotting (IB) with anti-KIT or anti-phosphotyrosine (pY) anti-bodies.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M., ... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences, 110(44), 17832-17837.
Figure 2 WT KIT activation is inhibited by anti-D4 antibodies.
Ba/F3 cells expressing WT KIT were plated in six-well platesand grown in the presence of 250 ng/mL SCF and different concentrations as indicated of Fab 79D.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M., ... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences, 110(44), 17832-17837.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-470CL | Human Anti-KIT Recombinant Antibody (TAB-470CL) | ELISA, WB | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-450CL | Afuco™ Anti-KIT Recombinant Antibody (AFC-450CL), ADCC Enhanced | ELISA, WB | Humanized IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-255 | Human Anti-KIT Recombinant Antibody (clone Fab19); Fab Fragment | WB, ELISA, FuncS | Human Fab |
HPAB-0019-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-0019-FY-F(E)) | FC | Humanized Fab |
HPAB-3398LY-F(E) | Human Anti-KIT Recombinant Antibody (clone 37M); Fab Fragment | ELISA, FC, WB | Human Fab |
HPAB-3399LY-F(E) | Human Anti-KIT Recombinant Antibody (clone 37C); Fab Fragment | ELISA, FC, WB | Human Fab |
HPAB-1951-FY-F(E) | Mouse Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-1951-FY-F(E)) | Block, ELISA, FC, IF | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-465LC | Human Anti-KIT Recombinant Antibody (TAB-465LC) | ELISA, SPR | Human antibody |
TAB-466LC | Human Anti-KIT Recombinant Antibody (TAB-466LC) | ELISA, SPR | Human antibody |
TAB-465LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-465LC-S(P)) | FC, WB, SPR, ELISA | Human scFv |
TAB-466LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-466LC-S(P)) | FC, WB, SPR, ELISA | Human scFv |
TAB-468LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-468LC-S(P)) | FC, WB, ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-467LC | Anti-Human KIT Recombinant Antibody (SR1) | WB | |
TAB-467LC-S(P) | Anti-Human KIT Recombinant Antibody scFv Fragment (SR1) | WB | |
TAB-467LC-F(E) | Anti-Human KIT Recombinant Antibody Fab Fragment (SR1) | WB |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0316MZ | Chicken Anti-KIT Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1629CQ | Recombinant Mouse Anti-KIT Antibody (A3C6E2) | FC, BL | IgG1, κ |
NEUT-1633CQ | Recombinant Rat Anti-Kit Antibody (ACK2) | FC, BL, Depletion | IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1630CQ | Recombinant Mouse Anti-KIT Antibody (CBL788) | WB, FC, CyTOF, ELISA(Cap), Neut | IgG1 |
NEUT-1631CQ | Recombinant Mouse Anti-KIT Antibody (14G5) | Neut, WB | IgG1 |
NEUT-1632CQ | Recombinant Mouse Anti-KIT Antibody (12A8) | WB, IP, FC, Neut | IgG1 |
NEUT-1634CQ | Recombinant Mouse Anti-KIT Antibody (CBL158) | Neut, WB | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1948 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (DS1948AB) | IHC-P, IHC-Fr | IgG |
MOR-4551 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH61DS) | WB, IF, ICC | IgG |
MOR-4552 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH62DS) | WB, IF, ICC, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0019-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-0019-FY-S(P)) | FC | Humanized scFv |
HPAB-N0156-YC-S(P) | Mouse Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-N0156-YC-S(P)) | ELISA, Inhib, FuncS | Mouse scFv |
HPAB-N0157-YC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-N0157-YC-S(P)) | ELISA, Inhib, FuncS | Humanized scFv |
HPAB-1949-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1949-FY-S(P)) | Block, ELISA, FC, IF | Humanized scFv |
HPAB-1950-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1950-FY-S(P)) | Block, ELISA, FC, IF | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-3398LY | Human Anti-KIT Recombinant Antibody (clone 37M) | ELISA, FC, WB | Human IgG |
HPAB-3399LY | Human Anti-KIT Recombinant Antibody (clone 37C) | ELISA, FC, WB | Human IgG |
HPAB-1951-FY | Mouse Anti-KIT Recombinant Antibody (HPAB-1951-FY) | ELISA, FC, Inhib, FuncS | Mouse IgG |
HPAB-1952-FY | Mouse Anti-KIT Recombinant Antibody (HPAB-1952-FY) | ELISA, Inhib | Mouse IgG |
ZG-1110F | Mouse Anti-KIT Recombinant Antibody (clone T595) | ICC, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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