This product is a recombinant human antibody that recognizes SIRPA. The antibody was purified by affinity chromatography.
Figure 1 Anti-Human SIRPA Antibody (TAB-453CT) in WB
Western blot analysis of TAB-453CT was performed by loading Human SIRPA Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg 0.3 μg 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-453CT (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 2 Anti-Human SIRPA Antibody (TAB-453CT) in DB
Dot Blot analysis of TAB-453CT was performed by coating with Human SIRPA Protein (His Tag).
TAB-453CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
Figure 3 Anti-Human SIRPA Antibody (TAB-453CT) in ELISA
ELISA analysis of TAB-453CT was performed by coating with Human SIRPA Protein (His Tag). Then blocked with BSA and incubated with TAB-453CT. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-1201-VHH | Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-454CT | Anti-Human SIRPA Recombinant Antibody (AM4-1) | ELISA | Human antibody |
TAB-454CT-S(P) | Anti-Human SIRPA Recombinant Antibody scFv Fragment (AM4-1) | ELISA | Human antibody |
TAB-454CT-F(E) | Anti-Human SIRPA Recombinant Antibody Fab Fragment (AM4-1) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-453CT-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (TAB-453CT-S(P)) | WB, ELISA | Human scFv |
TAB-453CT-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (TAB-453CT-F(E)) | WB, ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0465MZ | Chicken Anti-PTPNS1 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1970CQ | Recombinant Rat Anti-Sirpa Antibody (P84) | FC, BL, IF, IHC, IP | IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1971CQ | Recombinant Mouse Anti-Sirpa Antibody (CBL650) | WB, FC, CyTOF, Neut | IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4429 | Hi-Affi™ Recombinant Rabbit Anti-SIRPA Monoclonal Antibody (SI522DS) | ELISA | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0023-LSX | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1) | ELISA, Inhib | Humanized IgG |
HPAB-0024-LSX | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4) | ELISA | Humanized IgG |
HPAB-0025-LSX | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5) | ELISA, Inhib | Humanized IgG |
PABJ-0078 | Human Anti-SIRPA Recombinant Antibody (clone 218) | ELISA | Human IgG |
PABJ-0084 | Human Anti-SIRPA Recombinant Antibody (clone 119) | ELISA, Block | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0023-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); scFv Fragment | ELISA | Humanized scFv |
HPAB-0024-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); scFv Fragment | ELISA | Humanized scFv |
HPAB-0025-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5); scFv Fragment | ELISA | Humanized scFv |
HPAB-N0386-YC-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0386-YC-S(P)) | ELISA, FC | Human scFv |
HPAB-2559LY-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-2559LY-S(P)) | Inhib | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0023-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); Fab Fragment | ELISA | Humanized Fab |
HPAB-N0386-YC-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0386-YC-F(E)) | ELISA, FC | Human Fab |
HPAB-N0387-YC-F(E) | Mouse Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0387-YC-F(E)) | ELISA, FC | Mouse Fab |
HPAB-N0388-YC-F(E) | Mouse Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0388-YC-F(E)) | ELISA, FC | Mouse Fab |
HPAB-2559LY-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-2559LY-F(E)) | Inhib | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-080CN | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35) | ELISA, Inhib, FuncS | Human IgG |
NS-080CN-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); Fab Fragment | ELISA | Human Fab |
NS-080CN-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); scFv Fragment | ELISA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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