Rabbit Anti-CHRNA4 Recombinant Antibody (clone R03-1A5) (CAT#: VS3-FY2047)
This is a rabbit recombinant monoclonal antibody that detects human CHRNA4. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IP. For detection of human CHRNA4 using immunoprecipitation, please refer to clone R03-1A5.
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WB
Figure 1 Anti-CHRNA4 Antibody (VS3-FY2047) in WB.
The Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 expression in 3T3 lysates were detected using the anti-Nicotinic Acetylcholine Receptor alpha 4 by western blot assay.
WB
Figure 2 Anti-CHRNA4 Antibody (VS3-FY2047) in WB.
The Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 expression in Hela, CHO-K1, C6, Ramos lysates were detected using the anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human nicotinic acetylcholine receptor α4/CHRNA4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster CHRNA4
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R03-1A5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Epilepsy, Nocturnal Frontal Lobe, 1 and Autosomal Dominant Nocturnal Frontal Lobe Epilepsy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cholinergic Receptor Nicotinic Alpha 4 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 4; Cholinergic Receptor, Nicotinic, Alpha 4 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 4; NACRA4; Neuronal Nicotinic Acetylcholine Receptor Alpha-4 Subunit; Acetylcholine Receptor, Nicotinic, Alpha 4 (Neuronal)
- Gene ID
- 1137
- UniProt ID
- P43681
- Sequence Similarities
- Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
- Cellular Localization
- Cell membrane, Membrane, Postsynaptic cell membrane, Synapse
- Post Translation Modifications
- Glycosylation at Asn57, Asn174, and Asn107 (NX_P43681 [NX_P43681-1])
Modification sites at PhosphoSitePlus (P43681)
Glycosylation from GlyGen (P43681) 3 sites
- Protein Refseq
- NP_000735.1; NP_001243502.1
- Function
- After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CHRNA4"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3787z | Mouse Anti-CHRNA4 Recombinant Antibody (clone 19G3) | WB, ELISA | Mouse IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0698 | Hi-Affi™ Rabbit Anti-CHRNA4 Recombinant Antibody (clone DS698AB) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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