Rabbit Anti-CRK Recombinant Antibody (clone R83-7X-4) (CAT#: VS3-FY2131)

This is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CRK. It cross-reacts with mouse, rat. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Normal Tissue
RNA Expression

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat CRK
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R83-7X-4
  • Applications
  • Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 34kDa, while the observed molecular weight stands at 38kDa.
  • Related Disease
  • Sarcoma and Chromosome 17P13.3, Centromeric, Duplication Syndrome

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/50
    Flow Cytometry: 1/50

Target

  • Alternative Names
  • CRK Proto-Oncogene, Adaptor Protein; V-Crk Avian Sarcoma Virus CT10 Oncogene Homolog; Proto-Oncogene C-Crk; P38; V-Crk Sarcoma Virus CT10 Oncogene-Like Protein; Adapter Molecule Crk; CRKII
  • Sequence Similarities
  • Belongs to the CRK family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Membrane
  • Post Translation Modifications
  • Phosphorylated on Tyr-221 upon cell adhesion.
    Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain.
    This leads finally to the down-regulation of the Crk signaling pathway (PubMed:17515907).
    Isoform Crk-II: Phosphorylated by KIT ().
  • Function
  • Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.

Recommended Products

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Rabbit Monoclonal Antibody Products

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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