This product is a rabbit antibody that recognizes E2F1.
Figure 1 Rabbit Anti-E2F1 Antibody (ZG-0641J) in WB
Western Blot
Positive WB detected in: Jurkat whole cell lysate, PC-3 whole cell lysate, Rat Heart tissue
All lanes: E2F1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 60 kDa
Figure 2 Rabbit Anti-E2F1 Antibody (ZG-0641J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 3 Rabbit Anti-E2F1 Antibody (ZG-0641J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 4 Rabbit Anti-E2F1 Antibody (ZG-0641J) in IF
Immunofluorescence staining of Hela Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Figure 5 Rabbit Anti-E2F1 Antibody (ZG-0641J) in FC
Overlay histogram showing Hela cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-4001z | Mouse Anti-E2F1 Recombinant Antibody (clone 30B9) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
MOB-0156CT | Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) | ICC/IF, IP, WB | |
MRO-0508-CN | Rabbit Anti-E2F1 Recombinant Antibody (clone CBACN-206) | WB, IF, IHC, IP, FC | Rabbit IgG |
VS3-WK839 | Rabbit Anti-E2F1 Recombinant Antibody (VS3-WK839) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
VS3-FY466 | Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1080 | Hi-Affi™ Rabbit Anti-E2F1 Recombinant Antibody (clone DS1080AB) | WB, ICC, IF, IP, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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