This product is a rabbit antibody that recognizes FOXO3.
Figure 1 Rabbit Anti-FOXO3 Antibody (ZG-0676J) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate
All lanes: FOXO3A antibody at 1.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72, 49 KDa
Observed band size: 72-90 KDa
Figure 2 Rabbit Anti-FOXO3 Antibody (ZG-0676J) in IHC
IHC image of this product diluted at 1:180 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Rabbit Anti-FOXO3 Antibody (ZG-0676J) in IHC
IHC image of this product diluted at 1:180 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 4 Rabbit Anti-FOXO3 Antibody (ZG-0676J) in IF
Immunofluorescence staining of PC3 cells with this product at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1344 | Hi-Affi™ Rabbit Anti-FOXO3 Recombinant Antibody (clone DS1344AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-0602-CN | Rabbit Anti-FOXO3 Recombinant Antibody (clone SP0054) | WB, IF | Rabbit IgG |
ZG-0675J | Rabbit Anti-FOXO3 Recombinant Antibody (clone 1E1) | ELISA, WB, IHC | Rabbit IgG |
VS3-CJ151 | Rabbit Anti-FOXO3 Recombinant Antibody (VS3-CJ151) | WB, IHC | Rabbit IgG |
VS3-FY560 | Recombinant Rabbit Anti-FOXO3 Antibody (clone R03-2H2) | WB | Rabbit IgG |
VS3-FY561 | Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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