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Mouse Anti-HSPA8 Recombinant Antibody (clone 6C7) (CAT#: VS3-WK1578)

This antibody is a mouse recombinant antibody against human HSPA8, which functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. HSPA8 also functions as an ATPase in the disassembly of clathrin-coated vesicles during the transport of membrane components through the cell. The clone 6C7 can be used in WB analysis at a dilution of 1/500-2000, ELISA analysis at a dilution of 1/40000, and IHC analysis at a dilution of 1/100-300.

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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Figure 2 IHC staining of human testis
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/ihc_selected.jpg
Figure 3 IHC staining of human stomach
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/ihc_selected.jpg
Figure 4 IF staining of human cell line PC-3
Figure 4 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/987_G4_1_blue_red_green.jpg
Figure 5 IF staining of human cell line U-2 OS
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/802_G12_2_blue_red_green.jpg
Normal Tissue
Figure 6 Liver
Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_8_4.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8

Specifications

  • Immunogen
  • Synthesized peptide derived from the Internal region of human HSC 70.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human HSPA8
  • Species Reactivity
  • Human, Rat, Mouse
  • Clone
  • 6C7
  • Applications
  • WB, IHC-P, IF
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL
  • Buffer
  • PBS, 0.5% BSA, 50% Glycerol
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/2000
    IHC: 1/100-1/300
    ELISA: 1/40000

Target

  • Alternative Names
  • Heat Shock Protein Family A (Hsp70) Member 8; Lipopolysaccharide-Associated Protein 1; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; HSPA10; HSC70; HSP73; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Heat Shock Cognate 71 KDa Protein; Epididymis Luminal Protein 33
  • Long Name
  • Heat Shock Protein Family A (Hsp70) Member 8
  • Sequence Similarities
  • Belongs to the heat shock protein 70 family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Membrane, Nucleus, Spliceosome
  • Post Translation Modifications
  • Acetylated.
    ISGylated.
    Trimethylation at Lys-561 reduces fibrillar SNCA binding.
  • Function
  • Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HSPA8"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1697 Rabbit Anti-HSPA8 Recombinant Antibody (clone DS1697AB) WB, FC, IP, IHC Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC2078 PE-A68/Human HSPA8 (TVFDAKRLIGR) MHC Tetramer FCM
MHC-LC2079 APC-A68/Human HSPA8 (TVFDAKRLIGR) MHC Tetramer FCM
MHC-LC4316 PE-B*58:01/Human HSPA8 (KMKEIAEAY) MHC Tetramer FCM
MHC-LC4317 PE-A*29:02/Human HSPA8 (KMKEIAEAY) MHC Tetramer FCM
MHC-LC4318 PE-B*57:01/Human HSPA8 (KMKEIAEAY) MHC Tetramer FCM

Recombinant Antibody

CAT Product Name Application Type
MRO-0768-CN Rabbit Anti-HSPA8 Recombinant Antibody (clone CBACN-284) WB, IF, IHC, IP Rabbit IgG
VS3-QX561 Mouse Anti-HSPA8 Recombinant Antibody (clone 5C2-6G4-9A4) WB, IHC Mouse IgG1
VS3-QX562 Mouse Anti-HSPA8 Recombinant Antibody (clone 4C2-4A2-10E1) WB, IHC Mouse IgG1
VS3-QX563 Mouse Anti-HSPA8 Recombinant Antibody (clone 7G12) WB, IHC Mouse IgG1
VS3-WK1382 Mouse Anti-HSPA8 Recombinant Antibody (VS3-WK1382) WB, IHC-P Mouse IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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