Mouse Anti-MAPK7 Recombinant Antibody (clone 6D10) (CAT#: VS3-WK1614)
This antibody is a mouse recombinant antibody against human MAPK7, which is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. The clone 6D10 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31031/if_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31031/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/if_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_9_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_9_5.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_A_8_5.jpg
Figure 7 Skeletal muscle
(Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_3_6.jpg
(Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18561/41732_B_3_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, SuSa)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166484-MAPK7
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166484-MAPK7
Specifications
- Immunogen
- Synthesized peptide derived from human ERK5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAPK7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6D10
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- BMK1; ERK4; ERK5; PRKM7
- Gene ID
- 5598
- UniProt ID
- Q13164
- Long Name
- Mitogen-activated protein kinase 7
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity).
Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
- Protein Refseq
- NP_002740.2
- Function
- Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MAPK7"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2798z | Mouse Anti-MAPK7 Recombinant Antibody (clone 14C6) | WB, FC, ICC, IF | Mouse IgG1 |
| MRO-0549-CN | Recombinant Rabbit Anti-MAPK7 Monoclonal Antibody (CBACN-219) | WB, IF, IP, FC | Rabbit IgG |
| VS3-XY1042 | Mouse Anti-MAPK7 Recombinant Antibody (clone 8H1D5) | IHC | Mouse IgG1 |
| VS3-FY888 | Recombinant Mouse Anti-MAPK7 Antibody (clone 4D6) | IHC | Mouse IgG |
| VS3-FY889 | Recombinant Mouse Anti-MAPK7 Antibody (clone 4G5) | IHC-p, WB | Mouse IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0344MZ | Chicken Anti-MAPK7 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0345MZ | Chicken Anti-MAPK7 (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-0929MZ | Chicken Anti-ERK5 Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2158 | Hi-Affi™ Recombinant Rabbit Anti-MAPK7 Monoclonal Antibody (DS2158AB) | WB, ICC, FC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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