This product is a mouse antibody that recognizes MKI67.
Figure 1 Mouse Anti-MKI67 Recombinant Antibody (clone 4A8) (MOB-0066F) in IF
Immunofluorescence analysis of Hela cells. 1. Annexin VI polyclonal antibody (green) diluted 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor594 diluted 1:1000 (room temperature, 50 minutes).
Figure 2 Mouse Anti-MKI67 Recombinant Antibody (clone 4A8) (MOB-0066F) in IHC
Immunohistochemical analysis of paraffin-embedded human-uterine cancer tissue. 1. Ki 67 Monoclonal Antibody (4A8) 1:200 (4°C, overnight) dilution. 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
Figure 3 Mouse Anti-MKI67 Recombinant Antibody (clone 4A8) (MOB-0066F) in IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
Figure 4 Mouse Anti-MKI67 Recombinant Antibody (clone 4A8) (MOB-0066F) in IF
Immunofluorescence analysis of human breast cancer tissue. 1. Ki 67 Monoclonal Antibody (4A8) (red) is diluted at 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1072z | Mouse Anti-MKI67 Recombinant Antibody (clone 26F12) | WB, ELISA, IHC | Mouse IgG1 |
MRO-0899-CN | Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (CBACN-337) | WB, IHC, FC | Rabbit IgG |
HPAB-2357LY | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8) | IHC, ELISA, WB | Mouse IgG |
ZG-0240C | Mouse Anti-MKI67 Recombinant Antibody (ZG-0240C) | IHC, ELISA | Mouse IgG |
ZG-0241C | Mouse Anti-MKI67 Recombinant Antibody (ZG-0241C) | WB, ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2237 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) | ELISA, WB, IHC | IgG |
MOR-4638 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) | IF, ICC, FC, ChIP | IgG |
MOR-0054-FY | Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025) | ICC, IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-2357LY-F(E) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); Fab Fragment | IHC, ELISA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-2357LY-S(P) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); scfv Fragment | IHC, ELISA, WB | Mouse scfv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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