Rabbit Anti-NDUFAF4 Recombinant Antibody (clone R04-6A6) (CAT#: VS3-FY1788)
This R04-6A6 recombinant monoclonal antibody could potentially be used to target human NDUFAF4. The antibody is recommended for detection of NDUFAF4 protein by WB, ICC/IF, IP. Applications Tested: The NDUFAF4 antibody has been tested by western blot analysis of NDUFAF4 in K562, Hela lysates.
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WB
Figure 1 Anti-NDUFAF4 Antibody (VS3-FY1788) in WB.
The NDUFAF4 expression in K562, Hela lysates were detected using the anti-NDUFAF4 by western blot assay.
Specifications
- Immunogen
- Recombinant human NDUFAF4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NDUFAF4
- Species Reactivity
- Human
- Clone
- R04-6A6
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 20 kDa, while the observed molecular weight stands at 20 kDa.
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 15 and Mitochondrial Complex I Deficiency, Nuclear Type 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 4; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 4; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 4; Hormone-Regulated Proliferation-Associated Protein Of 20 KDa; C6orf66; HRPAP20; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 4
- Gene ID
- 29078
- UniProt ID
- Q9P032
- Sequence Similarities
- Belongs to the NDUFAF4 family.
- Cellular Localization
- Membrane, Mitochondrion
- Post Translation Modifications
- Phosphorylated on serine.
Prolactin stimulate serine phosphorylation ().
(Q9P032-NDUF4_HUMAN)
Ubiquitination at Lys51, Lys60, Lys66, Lys91, Lys97, and Lys107 (NX_Q9P032 [NX_Q9P032-1])
Modification sites at PhosphoSitePlus (Q9P032)
Glycosylation from GlyGen (Q9P032) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_054884.1
- Function
- Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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