Mouse Anti-PDGFRB Recombinant Antibody (clone muM4TS.11) (CAT#: HPAB-S0105-YC)

Figure 1 A. Effect of anti-PDGFRb antibodies on ¹²⁵I-labeled PDGF BB binding to PDGFRβ⁺ cells. B. Effect of anti-PDGFRβ antibodies on PDGF BB-mediated proliferation.

CHO C4 cells expressing the human PDGFRb were preincubated with 5 m g of the indicated antibody. Addition of 100 ng of unlabeled PDGF BB served to indicate the level of nonspecific ¹²⁵I-labeled PDGF BB binding. CHO C4 cells were preincubated with various concentrations of M4TS.11 (●), M4TS.15 (▲), or M4TS.22 (■) for 2 h, after which PDGF BB was added.

Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.

Figure 2 The M4TS.11 antibody impairs PDGF BB-mediated receptor dimerization.

Cells preincubated with M4TS.11 before exposure to ¹²⁵I-labeled PDGF BB and cross-linking had approximately 50% that of the level of receptor dimer as compared with cells exposed to PDGF BB in the presence M4TS.15 or no antibody.

Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.

Figure 3 Binding of the M4TS antibodies to PDGFRb domain deletion mutants.

Reactivity of M4TS.11, M4TS.15, and M4TS.22 with various PDGFRb deletion mutants was determined by ELISA. The PDGFRb extracellular domain composition of each mutant is indicated.

Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.

Figure 4 Effect of anti-PDGFRb antibodies on PDGF BB-mediated proliferation of baboon and rabbit cells.

Baboon SMC or the rabbit cornea cell line SIRC were preincubated with 10 μg/ml concentrations of the indicated antibodies for 2 h, after which PDGF BB was added.[3H]Thymidine incorporation was determined 20 h later. Proliferation of antibody-treated cells is expressed as a % of the level of proliferation observed with a nonbinding isotype-matched control antibody (Control Ab). It demonstrates that preincubation with either M4TS.11 or M4TS.22 significantly decreased PDGF BB-mediated proliferation of the baboon SMC. M4TS.11, but not M4TS.22, exhibited a similar effect on the rabbit SIRC cell line.

Shulman, T., Sauer, F. G., Jackman, R. M., Chang, C. N., & Landolfi, N. F. (1997). An antibody reactive with domain 4 of the platelet-derived growth factor β receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. Journal of Biological Chemistry, 272(28), 17400-17404.