The purified Rabbit Anti-SIRT1 Recombinant Antibody (clone 9E9) displayed high specificity against the SIRT1 protein of human.
Figure 1 Rabbit Anti-SIRT1 Antibody (ZG-0071U) in IHC
IHC image of ZG-0071U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 2 Rabbit Anti-SIRT1 Antibody (ZG-0071U) in IF
Immunofluorescence staining of Hela Cells with ZG-0071U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3980z | Mouse Anti-SIRT1 Recombinant Antibody (clone 4G3) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
MRO-1393-CN | Recombinant Mouse Anti-SIRT1 Monoclonal Antibody (7-C5-B2) | WB, IF, IHC | Mouse IgG2b |
MRO-1394-CN | Recombinant Rabbit Anti-SIRT1 Monoclonal Antibody (CBACN-504) | WB, IHC, IP | Rabbit IgG |
MRO-2188-CN | Rabbit Anti-SIRT1 Polyclonal Antibody (MRO-2188-CN) | WB, IHC, FC | Rabbit IgG |
ZG-087R | Mouse Anti-SIRT1 Recombinant Antibody (ZG-087R) | WB | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0777MZ | Chicken Anti-SIRT1 Polyclonal IgY | IHC, IF, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3267 | Hi-Affi™ Recombinant Rabbit Anti-SIRT1 Monoclonal Antibody (DS3267AB) | IP, WB | IgG |
MOR-0043-FY | Rabbit Anti-SIRT1 Recombinant Antibody (clone AFY0014) | ICC, IHC, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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