This product is a recombinant human antibody that recognizes TIGIT. The antibody is an effective blocker of the interaction between CD155 and TIGIT. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 1 Anti-TIGIT Recombinant Antibody (HPAB-0669YY) in SDS-PAGE
SDS-PAGE analysis of HPAB-0669YY in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.
Figure 2 Anti-TIGIT Recombinant Antibody (HPAB-0669YY) in WB
Western blot analysis of HPAB-0669YY was performed by loading Recombinant Human TIGIT (Lane 2, 0.3 μg and lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0669YY and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 3 Anti-TIGIT Recombinant Antibody (HPAB-0669YY) in ELISA
ELISA analysis of HPAB-0669YY was performed by coating with Recombinant Human TIGIT Protein. Then blocked with BSA, and incubated with Anti-Human TIGIT Antibody (HPAB-0669YY). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0279CL | Hamster Anti-TIGIT Recombinant Antibody (TAB-0279CL) | FuncS | Hamster IgG |
TAB-0280CL | Hamster Anti-TIGIT Recombinant Antibody (TAB-0280CL) | Inhib, WB, FC | Hamster IgG |
TAB-659CT-F(E) | Mouse Anti-TIGIT Recombinant Antibody; Fab Fragment (TAB-659CT-F(E)) | WB, ELISA, FC, Inhib | Mouse Fab |
TAB-660CT-F(E) | Mouse Anti-TIGIT Recombinant Antibody; Fab Fragment (TAB-660CT-F(E)) | WB, ELISA, FC, Inhib | Mouse Fab |
TAB-661CT-F(E) | Rat Anti-TIGIT Recombinant Antibody; Fab Fragment (TAB-661CT-F(E)) | WB, ELISA, FC, Inhib | Rat Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-662CT | Humanized Anti-TIGIT Recombinant Antibody (TAB-662CT) | WB, ELISA, FC, Inhib | Humanized IgG4 |
TAB-663CT | Humanized Anti-TIGIT Recombinant Antibody (TAB-663CT) | WB, ELISA, FC, Inhib | Humanized IgG4 |
TAB-662CT-S(P) | Humanized Anti-TIGIT Recombinant Antibody; scFv Fragment (TAB-662CT-S(P)) | WB, ELISA, FC, Inhib | Humanized scFv |
TAB-662CT-F(E) | Humanized Anti-TIGIT Recombinant Antibody; Fab Fragment (TAB-662CT-F(E)) | WB, ELISA, FC, Inhib | Humanized Fab |
TAB-663CT-F(E) | Humanized Anti-TIGIT Recombinant Antibody; Fab Fragment (TAB-663CT-F(E)) | WB, ELISA, FC, Inhib | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-2025CQ | Mouse Anti-TIGIT Recombinant Antibody (clone 1G9) | FC, FuncS | Mouse IgG1, κ |
NEUT-2026CQ | Mouse Anti-TIGIT Recombinant Antibody (clone CBL382) | Block | Mouse IgG1 |
NEUT-2027CQ | Mouse Anti-TIGIT Recombinant Antibody (clone CBL383) | Block, CyTOF, FC | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0019CQ | Mouse Anti-TIGIT Recombinant Antibody (HPAB-0019CQ) | ELISA, FC, Inhib | Mouse IgG2a, κ |
HPAB-0020CQ | Mouse Anti-TIGIT Recombinant Antibody (HPAB-0020CQ) | ELISA, FC, Inhib | Mouse IgG2a, κ |
HPAB-0021CQ | Mouse Anti-TIGIT Recombinant Antibody (HPAB-0021CQ) | ELISA, FC, FuncS | Mouse IgG2a, κ |
HPAB-0022CQ | Human Anti-TIGIT Recombinant Antibody (HPAB-0022CQ) | ELISA, FC, Block, FuncS | Humanized IgG1, κ |
HPAB-0023CQ | Human Anti-TIGIT Recombinant Antibody (HPAB-0023CQ) | ELISA, FC, Block, FuncS | Humanized IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0019CQ-S(P) | Mouse Anti-TIGIT Recombinant Antibody; scFv Fragment (HPAB-0019CQ-S(P)) | ELISA, FC | Mouse scFv |
HPAB-0020CQ-S(P) | Mouse Anti-TIGIT Recombinant Antibody; scFv Fragment (HPAB-0020CQ-S(P)) | ELISA, FC | Mouse scFv |
HPAB-0021CQ-S(P) | Mouse Anti-TIGIT Recombinant Antibody; scFv Fragment (HPAB-0021CQ-S(P)) | ELISA, FC | Mouse scFv |
HPAB-0906-YJ-S(P) | Human Anti-TIGIT Recombinant Antibody; scFv Fragment (HPAB-0906-YJ-S(P)) | ELISA, Block | Human scFv |
HPAB-0907-YJ-S(P) | Human Anti-TIGIT Recombinant Antibody; scFv Fragment (HPAB-0907-YJ-S(P)) | ELISA, Block | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0019CQ-F(E) | Mouse Anti-TIGIT Recombinant Antibody; Fab Fragment (HPAB-0019CQ-F(E)) | ELISA, FC | Mouse Fab |
HPAB-0020CQ-F(E) | Mouse Anti-TIGIT Recombinant Antibody; Fab Fragment (HPAB-0020CQ-F(E)) | ELISA, FC | Mouse Fab |
HPAB-0021CQ-F(E) | Mouse Anti-TIGIT Recombinant Antibody; Fab Fragment (HPAB-0021CQ-F(E)) | ELISA, FC | Mouse Fab |
HPAB-0022CQ-F(E) | Human Anti-TIGIT Recombinant Antibody; Fab Fragment (HPAB-0022CQ-F(E)) | ELISA, FC | Humanized Fab |
HPAB-0023CQ-F(E) | Human Anti-TIGIT Recombinant Antibody; Fab Fragment (HPAB-0023CQ-F(E)) | ELISA, FC, Block | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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