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  • Recombinant Mouse Anti-ENO1 Capture Antibody (clone 9F10)

Recombinant Mouse Anti-ENO1 Capture Antibody (clone 9F10) (CAT#: VS3-WK1658)

This antibody is a mouse recombinant antibody against human ENO1. The sandwich ELISA was established using matched antibody pair 9F10 and 6H2. The clone 9F10 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human ENO1 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2.

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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
Figure 3 IF staining of human cell line A549
Figure 3 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
Figure 4 IHC staining of human pancreas
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
Figure 5 IHC staining of human kidney
Figure 5 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
Figure 6 IHC staining of human kidney
Figure 6 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
Figure 7 IF staining of human cell line MCF7
Figure 7 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Figure 8 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
Figure 9 Colon
Figure 9 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
Figure 10 Liver
Figure 10 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
Figure 11 Pancreas
Figure 11 Pancreas
(Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
Figure 12 Kidney
Figure 12 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
Figure 13 Testis
Figure 13 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
Figure 14 Lymph node
Figure 14 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
RNA Expression
Figure 15 RNA cell line category: Low cell line specificity
Figure 15 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1

Specifications

  • Immunogen
  • Recombinant human ENO1 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2
  • Specificity
  • Human ENO1
  • Species Reactivity
  • Human
  • Clone
  • 9F10
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.09% sodium azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • ELISA: 1/1000

Target

  • Alternative Names
  • Enolase 1; 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; Alpha-Enolase; EC 4.2.1.11; ENO1L1; MPB1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1
  • Long Name
  • Enolase 1
  • Sequence Similarities
  • Belongs to the enolase family.
  • Cellular Localization
  • Cell membrane, Cytoplasm, Membrane, Nucleus
  • Post Translation Modifications
  • ISGylated.
  • Function
  • Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons.
    Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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TAB-350MZ Anti-Human ENO1 Recombinant Antibody (A3) WB, FC Human antibody
TAB-350MZ-S(P) Anti-Human ENO1 Recombinant Antibody scFv Fragment (A3) WB, FC Human antibody
TAB-350MZ-F(E) Anti-Human ENO1 Recombinant Antibody Fab Fragment (A3) WB, FC Human antibody

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