Recombinant Rabbit Anti-PDGFRB Detection Antibody (clone R39-2X-5) (CAT#: VS3-WK1905)

• Immunogen
• Recombinant human PDGFRB protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PDGFRB

• Species Reactivity
• Human

• Clone
• R39-2X-5

• Applications
• ELISA

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 0.01M PBS, pH 7.2

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Alternative Names
• Platelet Derived Growth Factor Receptor Beta; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; Beta-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor 1; CD140 Antigen-Like Family Member B; PDGF-R-Beta; EC 2.7.10.1; PDGFR-Beta; PDGFR-1; PDGFR1; PDGFR; Platelet-Derived Growth Factor Receptor Beta

• Gene ID
• 5159

• UniProt ID
• P09619

• Long Name
• Platelet Derived Growth Factor Receptor Beta

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

• Cellular Localization
• Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

• Post Translation Modifications
• Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-579, and to a lesser degree, at Tyr-581, is important for interaction with SRC family kinases. Phosphorylation at Tyr-740 and Tyr-751 is important for interaction with PIK3R1. Phosphorylation at Tyr-751 is important for interaction with NCK1. Phosphorylation at Tyr-771 and Tyr-857 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-857 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1009 is important for interaction with PTPN11. Phosphorylation at Tyr-1009 and Tyr-1021 is important for interaction with PLCG1. Phosphorylation at Tyr-1021 is important for interaction with CBL; PLCG1 and CBL compete for the same binding site. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. Dephosphorylated by PTPN2 at Tyr-579 and Tyr-1021.

• Protein Refseq
• NP_002600.1

• Function
• Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.

Capture antibody

• VS3-WK1904
• Recombinant Rabbit Anti-PDGFRB Capture Antibody (clone R11-4M-8)

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2RD-A1517-YJ
• Sheep Anti-Rabbit IgG-heavy and light chain Secondary antibody