Mouse Anti-BRDU Recombinant Antibody (clone BMG-6H8); Fab Fragment (CAT#: DrMAB-188)

Recombinant Mouse Antibody binds selectively to Mouse BrdU.


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  • Published Data
  • Datasheet
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IHC

Figure 1 Immunohistochemical analysis of BrdU retention in keratinocytes at various time points after labeling.

Figure 1 Immunohistochemical analysis of BrdU retention in keratinocytes at various time points after labeling.

Albert, M. R., Foster, R. A., & Vogel, J. C. (2001). Murine epidermal label-retaining cells isolated by flow cytometry do not express the stem cell markers CD34, Sca-1, or Flk-1. Journal of investigative dermatology, 117(4), 943-948.

FC

Figure 2 Flow cytometric analysis of cells double stained for BrdU and stem cell markers.

Figure 2 Flow cytometric analysis of cells double stained for BrdU and stem cell markers.

Albert, M. R., Foster, R. A., & Vogel, J. C. (2001). Murine epidermal label-retaining cells isolated by flow cytometry do not express the stem cell markers CD34, Sca-1, or Flk-1. Journal of investigative dermatology, 117(4), 943-948.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Type
  • Mouse Fab
  • Specificity
  • The antibody reacts with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU) and with cellular DNA that contains incorporated BrdU; it shows no crossreactivity with other cellular components. The antibody may be applied for the qualitative measurement of cell proliferation (BrdU incorporation) on a single-cell level, and for quantitative measurement in a cell population by ELISA.
  • Clone
  • BMG-6H8
  • Applications
  • ELISA, ICC, IHC, FC
  • Conjugate
  • HRP

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Buffer
  • Lyophilizate, stabilized
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The BrdU antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry, Flow Cytometry.

Target

  • Alternative Names
  • 5-BROMO-1-(2-DEOXY-BETA-D-RIBOFURANOSYL)URACIL; 5-BRDU; 5-BROMO DEOXYURIDINE; 5-BROMO-2"-DESOXYURIDINE; (+)-5-BROMO-2"-DEOXYURIDINE; 5-BROMO-2"-DEOXYURIDINE; 2"-DEOXY-5-BROMOROURIDINE; 2"-DEOXY-5-BROMOURIDINE; BUDR; BROMO2"-DEOXYURIDINE,5-; BRUDR; BROXURI

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Recombinant Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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