Recombinant Antibody Production Services in Cell-Free System

With extensive knowledge of the fields related to antibodies, Creative Biolabs is offering the most comprehensive services for antibody production. We established cell-free protein expression platform to support the development of antibodies and other therapeutics. We now provide recombinant antibody production in a cell-free systems services for our clients around the world, including E. coli cell-free systems, reticulocyte cell-free systems, wheat germ cell-free systems, insect cell-free systems, CHO cell-free systems as well as labeling of antibodies and antibody fragments with non-canonical amino acids service.

Cell-Free Recombinant Antibody Production Services in Creative Biolabs:

• Recombinant Antibody Production Service in E. coli Cell-Free System

Creative Biolabs can employ an efficient transcription/translation reaction to express and manufacture the functionally active antibodies, including scFv antibody fragments, Fab antibody fragments, and full-length antibodies. By scaling up or miniaturizing the in vitro synthesis processes, the target antibodies and antibody fragments can be synthesized in milligram quantities.

• Recombinant Antibody Production Service in Reticulocyte Cell-Free System

Rabbit reticulocyte lysate can be used by Creative Biolabs to make antibodies and antibody fragments. This system extensively explains the mechanisms of translation, co-translational modifications, protein translocation, and mammalian post-translational modifications. The lysed reticulocytes recovered from rabbits treated with phenylhydrazine are used to create the reticulocyte lysate. Micrococcal nuclease is used as a pre-treatment to extract endogenous mRNA from the lysed reticulocytes.

• Recombinant Antibody Production Service in Wheat Germ Cell-Free System

Wheat germ cell-free system becomes a crucial tool for producing antibodies and antibody fragments. Milligram amounts of antibodies and antibody fragments can be produced by Creative Biolabs using the wheat germ cell-free technology, along with the highly parallel synthesis of numerous distinct antibodies under a single set of reaction conditions.

• Recombinant Antibody Production Service in Insect Cell-Free System

Insect cell-free systems are based on insect cell extracts, which contain ribosomes, tRNAs, soluble enzymes, and components crucial for initiation and elongation. Amino acids, ribonucleoside triphosphates (NTPs), and an NTP-regenerating mechanism must be added to the systems. Creative Biolabs now offers the antibody production service in insect cell-free systems.

• Recombinant Antibody Production Service in CHO Cell-Free System

CHO cells are the most widely used expression host for recombinant antibodies. The advantages of using CHO cells as a production host are combined with the advantages of cell-free systems generally in the CHO cell-free system. Depending on the quantity of antibody required for additional analysis, Creative Biolabs can be carried out on a variable scale ranging from microliter to milliliter over a liter.

• Labeling of Antibodies and Antibody Fragments with Non-canonical Amino Acids Service

Since cell-free systems are not constrained by the potential cytotoxicity of amino acid analogs, they offer an effective way to label antibodies and antibody fragments with non-canonical amino acids. Target antibodies and antibody fragments can be co-translationally tagged with one or more non-canonical amino acids through Creative Biolabs' labeling of antibodies and antibody fragments with non-canonical amino acids service.

Reference

1. Yeo, Xin Yi, et al. "Potentials of neuropeptides as therapeutic agents for neurological diseases." Biomedicines 10.2 (2022): 343.

For research use only. Not intended for any clinical use.