Recombinant Human Antibody (MEDI7814) is capable of binding to C5, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-C5 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-C5 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 MEDI7814 inhibits fluorescently labelled C5a binding to C5aR1 and C5aR2 receptors.
Representative results showing that MEDI7814 (▲) inhibits Alexa Fluor 647 labelled human C5a (AF647 C5a) binding to human C5aR1 receptor, the isotype matched irrelevant control (■) did not inhibit C5a ligand binding.
Colley, C. S., Popovic, B., Sridharan, S., Debreczeni, J. E., Hargeaves, D., Fung, M., ... & Eghobamien, L. (2018, January). Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. In MAbs (Vol. 10, No. 1, pp. 104-117). Taylor & Francis.
Figure 2 MEDI7814 neutralizes C5a and C5adesArg responses in vitro.
Representative results showing that MEDI7814 (▲) neutralizes calcium mobilisation in HEK 293 expressing human C5aR1 receptor and Ga16 using 100 pM human C5a.
Colley, C. S., Popovic, B., Sridharan, S., Debreczeni, J. E., Hargeaves, D., Fung, M., ... & Eghobamien, L. (2018, January). Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. In MAbs (Vol. 10, No. 1, pp. 104-117). Taylor & Francis.
Figure 3 MEDI7814 is specific for human C5a and C5.
Representative results showing that MEDI7814 does not bind to related human complement proteins: C3a, C3, C4a and C4. Purified complement proteins were titrated into a DELFIA biochemical competition assay measuring MEDI7814 IgG binding to 1.2 nM biotinylated human C5a. MEDI7814 binds to human C5a (●) and C5 (■) as both proteins compete for binding to biotinylated C5a. No competition was observed with complement C3, C3a, C4 and C4a indicating that MEDI7814 does not bind these complement family members when tested at a concentration in excess of 1000- fold over the biotinylated C5a concentration.
Colley, C. S., Popovic, B., Sridharan, S., Debreczeni, J. E., Hargeaves, D., Fung, M., ... & Eghobamien, L. (2018, January). Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. In MAbs (Vol. 10, No. 1, pp. 104-117). Taylor & Francis.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1366CL | Mouse Anti-C5 Recombinant Antibody (TAB-1366CL) | ELISA, Inhib | Mouse IgG |
TAB-1367CL | Mouse Anti-C5 Recombinant Antibody (TAB-1367CL) | ELISA, Inhib | Mouse IgG |
TAB-1368CL | Mouse Anti-C5 Recombinant Antibody (TAB-1368CL) | ELISA | Mouse IgG |
TAB-1370CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1370CL-S(P)) | ELISA | Mouse scFv |
TAB-1371CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1371CL-S(P)) | Inhib, FuncS | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1372CL | Human Anti-C5 Recombinant Antibody (TAB-1372CL) | Inhib, FuncS | Humanized antibody |
TAB-1373CL | Human Anti-C5 Recombinant Antibody (TAB-1373CL) | IB, CDC, Inhib | Humanized IgG |
TAB-1374CL | Human Anti-C5 Recombinant Antibody (TAB-1374CL) | ELISA, CDC, Inhib | Humanized IgG |
TAB-1375CL | Human Anti-C5 Recombinant Antibody (TAB-1375CL) | CDC, Inhib | Humanized IgG |
TAB-1376CL | Human Anti-C5 Recombinant Antibody (TAB-1376CL) | CDC, Inhib | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-082CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL249) | ELISA, Neut | Mouse IgG2a |
NEUT-083CQ | Mouse Anti-C5 Recombinant Antibody (clone MM0608-6H13) | ELISA, Neut, WB | Mouse IgG1 |
NEUT-084CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL030) | ELISA, WB, Neut | Mouse IgG1 |
NEUT-085CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL250) | ELISA, WB, IHC, FuncS | Mouse IgG1 |
NEUT-086CQ | Mouse Anti-C5 Recombinant Antibody (clone G25/2) | ELISA, IP, WB, Neut | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-088CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL771) | WB, Block, ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4104 | Hi-Affi™ Rabbit Anti-C5 Recombinant Antibody (clone SI175DS) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-453CQ | Anti-Human C5 Recombinant Antibody (Tesidolumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, λ2 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0609-CN-F(E) | Human Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0609-CN-F(E)) | ELISA, Block | Humanized Fab |
HPAB-0611-CN-F(E) | Rabbit Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0611-CN-F(E)) | WB, ELISA, FC | Rabbit Fab |
HPAB-0612-CN-F(E) | Human Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0612-CN-F(E)) | WB, ELISA, FC | Humanized Fab |
HPAB-0656LY-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0656LY-F(E)) | IP, RIA, ELISA, WB | Mouse Fab |
HPAB-0657LY-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0657LY-F(E)) | IP, RIA, ELISA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0127CQ-S(P) | Human Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0127CQ-S(P)) | ELISA, WB, Inhib, FuncS | Human scFv |
HPAB-M0017-YC-S(P) | Rabbit Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-M0017-YC-S(P)) | ELISA | Rabbit scFv |
HPAB-M0018-YC-S(P) | Human Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-M0018-YC-S(P)) | ELISA | Humanized scFv |
HPAB-0080-WJ-S(P) | Human Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0080-WJ-S(P)) | ELISA, FC | Humanized scFv |
HPAB-1577WJ-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (clone 5G1.1) | ELISA, WB | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABC-559 | Recombinant Llama Anti-C5 Single Domain Antibody (Nb36) | ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-453CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody (Tesidolumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-008 | Afuco™ Anti-C5 ADCC Recombinant Antibody (Eculizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-436CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody (Olendalizumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1029-FY | Human Anti-C5 Recombinant Antibody (HPAB-1029-FY) | ELISA | Human IgG1 |
HPAB-1031-FY | Human Anti-C5 Recombinant Antibody (HPAB-1031-FY) | ELISA | Human IgG1 |
HPAB-1032-FY | Human Anti-C5 Recombinant Antibody (HPAB-1032-FY) | ELISA | Human IgG1 |
HPAB-1033-FY | Human Anti-C5 Recombinant Antibody (HPAB-1033-FY) | ELISA | Human IgG1 |
HPAB-1034-FY | Human Anti-C5 Recombinant Antibody (HPAB-1034-FY) | ELISA | Human IgG2 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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