According to recent researches about coronavirus nucleoproteins (NPs or N proteins), Creative Biolabs now provides SARS-CoV-2 Nucleoprotein (NP) ELISA Kit for supporting SARS-CoV-2 detection and researches. Coronaviruses are enveloped viruses with a positive-sense RNA genome and a nucleocapsid of helical symmetry. Coronavirus NPs are required during the coronavirus RNA synthesis, and have RNA chaperone activity that may be involved in template switch. NP is the most abundant protein of coronavirus. During virion assembly, N protein can bind to viral RNA and lead to the formation of the helical nucleocapsid. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus can be used as a detective tool.
Principles of SARS-CoV-2 Nucleoprotein (NP) ELISA Kit
The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique, shown in Fig.1. This kit is to detect the novel coronavirus nucleocapsid (N) antigen in serum or plasma samples from candidates. In the beginning, a monoclonal antibody specific for SARS-CoV-2 NP has been pre-coated onto well plate strips. When samples are added to the plate, SARS-CoV-2 NP will be bound by the immobilized antibody if they are present in samples. After incubation the plate, it will be washed and a horseradish peroxidase-conjugated anti-SARS-CoV-2 NP antibody is added, producing an antibody-antigen-antibody "sandwich complex". Finally, washing unbound antibody by a TMB substrate solution and then color develops in proportion to the amount of SARS-CoV-2 NP bound and can be measured at 450 nm to observe test results.
Figure.1 Schematics of SARS-CoV-2 Nucleoprotein (NP) ELISA Kit.
Strengths of SARS-CoV-2 Nucleoprotein (NP) ELISA Kit
At Creative Biolabs, we provide the ELISA kit for supporting the detections and researches of SARS-CoV-2. Creative Biolabs offers efficient and high-quality products at an affordable price for our customers all over the world. At the same time, there is the shortest delivery cycle and help customers to reduce the period of research projects and increase efficiency. Creative Biolabs teams will work with partner customers to discuss and define specific requirements and expectations to meet the partnership objectives. If you want to know more information about the SARS-CoV-2 ELISA kit or other detection kits for SARS-CoV-2, such as detection kit (Colloidal Gold Method), fluorescent RT-PCR detection kit, and SARS-CoV-2 antibodies for researches, please feel free to contact us.
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