Intrabody Products

Intrabody: A Versatile Therapeutic Strategy

Intrabodies are antibodies or antibody fragments designed to function within cells, binding to specific intracellular proteins. Equipped with specific localization signals, they can be directed to various subcellular compartments. Their excellent antigen specificity and affinity make them valuable biotechnological tools. By disrupting, modulating, or investigating the functions of intracellular proteins, intrabodies have found applications in diverse fields such as cancer research, HIV, autoimmune diseases, neurodegenerative disorders, and transplantation.

The Molecular Mechanism of Intrabodies

• Preventing target protein's localization to its normal subcellular compartment and subsequent biological activity by sequestering.
• Targeting specific a protein to induce its degradation by the ubiquitin-proteasome pathway.
• Acting apoptotic pathway to trigger cell death.
• Through either sterically hindering the active site or allosterically altering its conformation to modulate enzyme function
• Interfering the normal interactions between proteins with other proteins or DNA to hinder biological signal pathways.

Fig.1 Phage display technique to produce intrabodies for cancer treatment.¹

Intrabody Expression and Purification Service at Creative Biolabs

Creative Biolabs specializes in providing a cost-effective intrabody expression and purification service to facilitate the development of innovative intrabody-based therapeutics. Our comprehensive, end-to-end offerings encompass every critical stage, from intrabody sequence optimization and gene synthesis to vector construction, expression, purification, and characterization of the intrabodies.

We implement advanced strategies to enhance the stability and solubility of intrabodies, ensuring optimal performance and therapeutic efficacy. Simultaneously, we leverage a variety of eukaryotic expression systems, such as CHO and HEK293, to ensure optimal protein expression, folding, and post-translational modifications, leading to the production of highly functional intrabodies. Moreover, our state-of-the-art purification techniques and analytical techniques enable the exceptional quality of produced intrabodies. By combining these capabilities, we are committed to delivering customized premium intrabodies to empower global customers' meaningful projects.

Fig.2 Our service process.

Available Intrabody Formats at Creative Biolabs

Our service supports a range of intrabody formats expression and purification, which include:

• Full-length IgG
• Antibody fragments
• single domain intrabodies
• bispecific tetravalent intradiabodies

Strategies for Enhancing Intrabody Stability and Solubility

• Library-Based Approaches:
  • Phage Display to select intrabodies with high affinity, specificity, and stability.
  • Yeast Display to select intrabodies based on factors like expression level, solubility, and binding affinity.
  • Ribosome Display to select intrabodies directly from mRNA, bypassing the need for protein folding and assembly
• Computational Design:
  • Analyzing the three-dimensional structure of the target antigen and the antibody fragment to identify potential interactions and design mutations to improve binding affinity, stability, and solubility.
  • Incorporating amino acid sequences that are likely to confer stability and solubility into the intrabody through mutagenesis.
• Directed Evolution:
  • Introducing random mutations into the antibody gene or recombining DNA fragments from different parental genes to directly evolve a better intrabody.

Highlight Features

• Flexible-scale intrabody production.
• industry regulatory specifications-compliant operation environment and process.
• Highly effective manufacturing techniques ensure prompt delivery.
• Exceptional intradodies production in quality and quantity.

As a leader in antibody production, Creative Biolabs has always remained true to our original aspirations and is committed to producing high-quality and efficient antibodies to serve the vast number of researchers. If you are looking for a reliable intrabody expression and purification service provider or want to get more details about our intrabody products, please contact us for a quote.

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For Research Use Only. Not For Clinical Use.