Anti-N. meningitidis subtype 9 Recombinant Antibody (5F81A4P1.9) (MRO-153MZ) (CAT#: MRO-153MZ)

Figure 1 The immunoglobulin class and subclass of the MAb of cell line 5F81A4 was characterized by ELISA as being IgG (Ig2a, kappa light chain).

This MAb was assessed with a Dot-Blot assay for specificity to reference strain P1.9 (M982) (Figure.1:2g) and to case strains N368/88, N398/92, N16/92, N17/92, N23/92, N35/92, N402/92, N403/92, and P1.9 (Figure.1:1a,b), using culture supernatant and ascites fluid, respectively.

Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

Figure 2 The immunoglobulin class and subclass of the MAb of cell line 5F81A4 was characterized by ELISA as being IgG (Ig2a, kappa light chain).

This MAb bound by immunoblot (Figure.1:4) to the class 1 protein, when 0.25% of Empigen BB detergent was added to restore the antibody binding activity to this class 1 subtype N368/88 protein (Figure.1:4a), N16/92 (4c), N 23/92 (4e).

Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

Figure 3 The ability of the 5F81A4 MAb to bind to 46 kDa protein on the surface ofthe pathogenic N. meningitidis group B strain was also determined by indirect immunofluorescence assay and was analyzed by flow cytometry.

The B:4:P1.9 strain of N. meningitidis was used.

Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil