Anti-P. aeruginosa lipopolysaccharide Recombinant Antibody (S20) (MRO-155MZ) (CAT#: MRO-155MZ)

Figure 1 Blocking of the binding of human anti-LPS MAb S20 to solid-phase P. aeruginosa strain 06ad LPS O-side-chain PS by preincubation with 06ad PS (▪) but not control PS from Streptococcus pneumoniae serotype 6B (♦) in an ELISA.

The x axis shows micrograms of 06ad PS or control PS per milliliter added to each reaction tube. The y axis depicts the percent reduction of absorbance obtained with the PS inhibitor. Data represent means from three separate assays, each run in duplicate, and standard errors of the means.

Human Monoclonal Antibodies against Pseudomonas aeruginosa Lipopolysaccharide Derived from Transgenic Mice Containing Megabase Human Immunoglobulin Loci Are Opsonic and Protective against Fatal Pseudomonas Sepsis

Figure 2 Human MAb S20 binds only to P. aeruginosa 06ad LPS O-side-chain PS and not to LPS O-side-chain PSs from other strains.

The x axis depicts the serotype of pseudomonas O-side-chain PS used to coat the ELISA plate, while the y axis indicates the absorbance (OD, optical density). Bars represent the mean absorbance from triplicate wells in one representative assay.

Human Monoclonal Antibodies against Pseudomonas aeruginosa Lipopolysaccharide Derived from Transgenic Mice Containing Megabase Human Immunoglobulin Loci Are Opsonic and Protective against Fatal Pseudomonas Sepsis

Figure 3 Opsonization of FITC-labeled P. aeruginosa 06ad by human MAb S20 for uptake by fresh human PMN in the presence of human complement (serum from an agammaglobulinemic patient).

Opsonization and phagocytosis by PMN are shown as mean fluorescence obtained by flow cytometry gated for human PMN as described in Materials and Methods. The results shown are from one representative experiment out of four separate experiments, all with similar results. MOPC, MOPC 21.

Human Monoclonal Antibodies against Pseudomonas aeruginosa Lipopolysaccharide Derived from Transgenic Mice Containing Megabase Human Immunoglobulin Loci Are Opsonic and Protective against Fatal Pseudomonas Sepsis