Products

CBGlyco™ Activated glutaraldehyde HRP Labeling Kit (Glyco-034CL)

Activated Peroxidase and Antibody Labeling Kit is designed to prepare and purify HRP-conjugated antibodies with any previously unmodified primary or secondary antibody (IgG).      

Specifications
Product Size
5 reactions
Label or Dye
HRP (Horseradish Peroxidase)
Labeling Scale
300 µg
Labeling Target
Antibodies (IgG)
Contents & storage
Sufficient For: 5 conjugations, each with 0.3 mg antibody (IgG)
• Activated Peroxidase, 5 mg
• Conjugation Buffer, 50 mL
• Lysine, 250 mg
• Protein A/G Column, 0.5 mL
• Gentle Ag/Ab Binding Buffer, 200 mL
• Gentle Ag/Ab Elution Buffer, 200 mL

Store activated peroxidase at -20°C. Store other components at 4°C.
BACKGROUND
Description
Activated Peroxidase is HRP enzyme that has been activated with glutaraldehyde to contain reactive groups that will spontaneously conjugate with amines on an antibody (or other protein). Incubation of this Activated Peroxidase with IgG in sodium carbonate buffer (pH 9.5) results in formation of a conjugate where both the enzyme activity of HRP and the antigen-binding activity of IgG are preserved. The Antibody Labeling Kit includes the Activated Peroxidase and a Protein A/G column and buffers to purify the antibody conjugate from excess, unreacted HRP enzyme.

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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