Ankyrin repeat scaffold protein anti-Human α-Na,K-ATPase (CAT#: SAR-L043)
The Ankyrin repeat that bound α-Na,K-ATPase was isolated from the library using Phage display techonology. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops, first discovered in signaling proteins in yeast Cdc10 and Drosophila Notch. Domains consisting of ankyrin repeats mediate protein–protein interactions and are among the most common structural motifs in known proteins. After affinity maturation, Ankyrin repeat variants were identified having affinities (KD=10-25 nM) for α-Na,K-ATPase as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Specifications
- Scaffold Name
- Ankyrin repeat
- Origin
- Ankyrin repeat proteins
- Core Structure
- 2α-helices, β-turn
- Variable Regions
- b-turn,1a-helix and loop
- Target
- α-Na,K-ATPase
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 10-25 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Ankyrin repeat proteins; Ankyrin repeat; α-Na,K-ATPase; Na+/K+ pump; sodium-potassium pump
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
Customer Reviews and Q&As
There are currently no Customer reviews or questions for SAR-L043. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.