Products

CBGlyco™ Hydrazide-Biotin (Glyco-025CL)

Hydrazide-Biotin is the shortest and simplest hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.
This biotin hydrazide reagent enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.      

Specifications
Product Size
100 mg
Label or Dye
Biotin
Spacer
Short
Solubility
DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Cell Permeability
Cell-Permeant
Chemical Reactivity
Carboxylic Acid, Ketone, Aldehyde
Contents & storage
Store at 4°C.
BACKGROUND
Description
This biotin hydrazide reagent enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies because biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.
Properties

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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