7-OHC(7-hydroxycoumarin) & AP (alkaline phosphatase)

Umbelliferone, also known as 7-hydroxycoumarin, hydrangine, skimmetine, and beta-umbelliferone, is a widespread natural product of the coumarin family. The ultraviolet activity of umbelliferone led to its use as a sunscreen agent, and an optical brightener for textiles. It has also been used as a gain mediumfor dye lasers. Umbelliferone can be used as a fluorescence indicator for metal ions such as copper and calcium.

Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, intestinal mucosa and the placenta. It is widely used in diagnostics.