PYCARD

Intracellular

Cell type enhanced (Kupffer cells, Proximal enterocytes, Hofbauer cells, Paneth cells, Macrophages)

Group enriched (neutrophil, non-classical monocyte, intermediate monocyte, classical monocyte, eosinophil, myeloid DC, plasmacytoid DC, basophil)

Cell line enhanced (MCF7, NB-4, THP-1, U-937)

Self-associates; enforced oligomerization induces apoptosis, NF-kappa-B regulation and interleukin-1 beta secretion (PubMed:15641782, 17599095, 33420028, 33420033). Homooligomers can form disk-like particles of approximately 12 nm diameter and approximately 1 nm height (PubMed:15641782, 17599095). Next to isoform 1, also isoform 2 and isoform 3 may be involved in oligomerization leading to functional regulation (Probable). Component of several inflammasomes containing one pattern recognition receptor/sensor, such as NLRP1, NLRP2, NLRP3, NLRP6, NLRC4, AIM2, MEFV or NOD2, and probably NLRC4, NLRP12 or IFI16 (PubMed:11374873, 12191486, 15030775, 15456791, 19158676, 30674671, 34678144). Major component of the ASC pyroptosome, a 1-2 um supramolecular assembly (one per macrophage cell) which consists of oligomerized PYCARD dimers and CASP1 (PubMed:17599095). Interacts with CASP1 (precursor form); the interaction induces activation of CASP1 leading to the processing of interleukin-1 beta; PYCARD competes with RIPK2 for binding to CASP1 (PubMed:11967258, 14634131, 16585594, 17599095, 33420033). Interacts with NLRP3; the interaction requires the homooligomerization of NLRP3 (PubMed:11786556, 15030775, 15020601, 34341353). Interacts with NLRP2, NLRC4, MEFV, CARD16, AIM2, IFI16, NOD2, DDX58, RIPK2, PYDC1, PYDC2, NLRP10, CASP8, CHUK, IKBKB and BAX (PubMed:11374873, 11498534, 12486103, 12646168, 12656673, 15456791, 14730312, 15096476, 17178784, 17339483, 18362139, 19158675, 19158676, 19915568, 21575908). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity).