Mouse Anti-ADIPOQ Recombinant Antibody (clone M24) (CAT#: NS-003CN)

Figure 1 Standard curve for ELISA assays using mAb Adiponectin M24 and three anti-adiponectin polyclonal antibodies.

Examples are provided of an ELISA standard curve using the reference standard adiponectin1-244 (C-term His tag) 0.0195 to 10 ng/well for the adiponectin globular domain pAb (FIG. a), and (FIG. b) for the N-terminal domain pAb. FIG. c is an example of a standard curve using the reference standard adiponectin 16-247 (N-term FLAG tag) for the C-terminal domain adiponectin pAb. The mAb is Adiponectin-M24 at 1 ug/ml.

Figure 2 Analysis of human plasma samples for adiponectin levels using mAb adiponectin M24 with globular, N-terminal and C-terminal adiponectin polyclonal antibodies.

Human fasting plasma samples collected from normal, diabetic, impaired glucose tolerant (IGT), impaired fasting glucose (IFG) and both IGT & IFG individuals was assayed for levels of adiponectin. The antibody pairs for assaying were mAb adiponectin M24 in combination with globular adiponectin (FIG. a), N-terminal (FIG. b) or C-terminal (FIG. c) adiponectin polyclonal antibodies. The adiponectin levels (ug/ml) are plotted against the levels of fasting blood glucose (mg/dL). Individuals with normal fasting glucose levels-diamond, IGT-square, IFG-triangles, IGT+IFG-cross, and diabetic-asterisk. A significant correlation is apparent, especially with the globular and N-terminal antibodies.