Recombinant Mouse Anti-B. burgdorferi OspA Antibody ( LA-2) (CAT#: PABZ-012)
Recombinant Mouse Antibody ( LA-2) is capable of binding to B. burgdorferi OspA, expressed in Chinese Hamster Ovary cells (CHO). This murine monoclonal antibody defines an important protective B-cell epitope of OspA against which protective sera have strong levels of reactivity.
Specific Inquiry
Figure 1 Western blot analysis of B. burgdorferi-specific antibodies.
(A) mAbs, IS, and NMS used for reconstitution of scid mice, tested on strains B31 and ZS7 of B. burgdorferi. (B) Sera from individual scid mice reconstituted with the respective mAbs, IS, or NMS, tested on strain ZS7 (strain used for infection of mice) and on strain B31. The following mAbs or sera were used: mAb LA-5 (lane 1), mAb LA-2 (lane 2), mAb LA-10 (lane 3), mAb LA-21 (lane 4), anti-B31 IS (lane 5), anti-ZS7 IS (lane 6) and NMS (lane 7). (C) Amount (Ag/ml) of B. burgdorferi-specific antibody(ies) in reconstituted scid mice during the observation period (one representative experiment). Curves: 1, mAb LA-10; 2, mAb LA-21; 3, mAb LA-2; 4, mAb LA-5; 5, anti-B31; 6, PBS.
Schaible, U. E., Kramer, M. D., Eichmann, K., Modolell, M., Museteanu, C., & Simon, M. M. (1990). Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proceedings of the National Academy of Sciences, 87(10), 3768-3772.
Figure 2 Histopathology in scid mice simultaneously inoculated s.c. with B. burgdorferi and i.p. with mAb LA-5 (a and c) or mAb LA-2 (b and d).
(a) Tibiotarsal joint of a scid mouse treated with LA-5. (b) Tibiotarsal joint of a scid mouse treated with the mAb LA-2. (c) Heart of a scid mouse treated with mAb LA-5. (d) Heart of a scid mouse treated with mAb LA-2.
Schaible, U. E., Kramer, M. D., Eichmann, K., Modolell, M., Museteanu, C., & Simon, M. M. (1990). Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proceedings of the National Academy of Sciences, 87(10), 3768-3772.
Specifications
- Immunogen
- B. burgdorferi Outer surface protein A
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native B. burgdorferi OspA
- Species Reactivity
- B. burgdorferi
- Clone
- LA-2
- Applications
- WB 1:1000-1:5000
FuncS Assay-Dependent
IF 1:100-1:500
Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody LA-2 has been reported in applications of WB, FuncS, IF, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- Outer surface protein A; OspA; Outer-Surface Protein A; OSPA
- Gene ID
- 8319333
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody (mAb184.1) | Neut | IgG |
| MRO-017CQ | Recombinant Mouse Anti-B. burgdorferi OspA Antibody (0551) | ELISA, IF | IgG |
| FAMAB-0187JF | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24) | WB, ELISA, IHC | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody Fab Fragment (mAb184.1) | Neut | IgG |
| PFBZ-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody Fab Fragment ( LA-2) | Neut, WB | IgG |
| FAMAB-0187JF-F(E) | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24); Fab Fragment | FACS, IF, WB, ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody scFv Fragment (mAb184.1) | Neut | scFv |
| PSBZ-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody scFv Fragment ( LA-2) | Neut, WB | scFv |
| FAMAB-0187JF-S(P) | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24); scFv Fragment | FACS, IF, WB, ELISA, FuncS | Human scFv |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0040CQ | Recombinant Mouse Anti-B. burgdorferi ospA Antibody (LA-2) | ELISA | IgG2b |
| EPAF-0041CQ | Recombinant Mouse Anti-B. burgdorferi ospA Antibody (105.5) | WB | IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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