Anti-CD20 Antibody-Cytokine Fusion Protein, IgG (DI-Leu16)-IL2 (CAT#: ACFP-SH134)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD20 IgG to IL2. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD20 as well as the biological activity of IL2. In-vitro studies of DI-Leu16-IL2 have demonstrated CD20 targeting, IL2 bioactivities, and antibody-dependent cell-mediated cytotoxicity (ADCC) functions. In a SCID mouse model, DI-Leu16-IL2 showed superior anti-lymphoma effects over rituximab or rituximab combined with systemic IL2. This immunocytokine was designed for treating Non-Hodgkin Lymphoma.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg
Figure 4 Colon
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg
Figure 5 Bone marrow
(Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg
(Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg
Figure 6 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg
Figure 7 RNA cell line category: Group enriched (Daudi, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1
Specifications
- Target
- CD20
- Molecule Class
- L Kappa; IgG1-IL2
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- DI-Leu16
- Related Disease
- Non-Hodgkin Lymphoma
- Cytokine 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- MS4A1; membrane-spanning 4-domains; subfamily A; member 1; B1; S7; Bp35; CD20; CVID5; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
- Gene ID
- 931
- UniProt ID
- A0A024R507
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MS4A1"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-913-sdAb | Recombinant Anti-human MS4A1 VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
| HPAB-M0704-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0704-YC) | ELISA, FuncS | Camelid VHH |
| HPAB-M0705-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0705-YC) | ELISA | Camelid VHH |
| HPAB-M0706-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0706-YC) | ELISA | Camelid VHH |
| HPAB-M0707-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0707-YC) | ELISA | Camelid VHH |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-133 | Anti-Human CD20 Recombinant Antibody (Afutuzumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | IgG3 - kappa |
| TAB-537CL | Human Anti-MS4A1 Recombinant Antibody (TAB-537CL) | ELISA | Human IgG |
| TAB-1635CL | Anti-Human MS4A1 Recombinant Antibody (BVX20) | Inhib | Humanized antibody |
| TAB-1636CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1636CL) | ADCC, CDC | Humanized IgG |
| TAB-1637CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1637CL) | ADCC, CDC | Humanized IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-419 | Human Anti-MS4A1 Recombinant Antibody (clone 2F2); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| HPAB-0018-FY-F(E) | Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-0018-FY-F(E)) | FC | Humanized Fab |
| HPAB-0021-FY-F(E) | Human Anti-MS4A1 Recombinant Antibody (clone hu3D9); Fab Fragment | FC | Humanized Fab |
| HPAB-0819-CN-F(E) | Human Anti-MS4A1 Recombinant Antibody (clone 1F5RKF12); Fab Fragment | ELISA, FC | Humanized Fab |
| HPAB-0820-CN-F(E) | Human Anti-MS4A1 Recombinant Antibody (clone RHAxRKF12); Fab Fragment | ELISA, FC | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1703CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1703CL) | FC, CDC, IP | Human IgG1, κ |
| TAB-1704CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1704CL) | IP | Human IgG1, κ |
| TAB-1705CL | Human Anti-MS4A1 Recombinant Antibody (TAB-1705CL) | Cyt, FC, FuncS | Human IgG3, κ |
| TAB-0001CL-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-0001CL-S(P)) | ELISA | Human scFv |
| PABX-028-F (E) | Recombinant Human Anti-CD20 Antibody Fab Fragment (Ofatumumab) | WB, ELISA, IF, FuncS | Fab |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1665CL-S(P) | Mouse Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1665CL-S(P)) | CDC | Mouse scFv |
| TAB-1666CL-S(P) | Mouse Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1666CL-S(P)) | CDC | Mouse scFv |
| TAB-1667CL-S(P) | Mouse Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1667CL-S(P)) | CDC | Mouse scFv |
| TAB-1668CL-S(P) | Mouse Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1668CL-S(P)) | CDC | Mouse scFv |
| TAB-1669CL-S(P) | Mouse Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1669CL-S(P)) | CDC | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1701CL-S(P) | Mouse Anti-MS4A1 Recombinant Antibody; scFv Fragment (TAB-1701CL-S(P)) | FuncS | Mouse scFv |
| TAB-1701CL-F(E) | Mouse Anti-MS4A1 Recombinant Antibody; Fab Fragment (TAB-1701CL-F(E)) | FuncS | Mouse Fab |
| PABX-026-S (P) | Recombinant Mouse Anti-CD20 Antibody scFv Fragment (C2H7) | WB, ELISA, FuncS | scFv |
| PABX-028-S (P) | Recombinant Human Anti-CD20 Antibody scFv Fragment (Ofatumumab) | WB, ELISA, IF, FuncS | scFv |
| TAB-007ML | Anti-Canine MS4A1 Recombinant Antibody (Blontuvetmab) | ELISA, IHC, FC, IP, IF, FuncS | IgG2, κ, λ |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-134LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-135LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-136LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-137LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-138LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
Low- or Non-fucosylated Oligosaccharide
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-134LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-135LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-136LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-137LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-138LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
Hyper-galactosylated
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-142LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Hyper-galactosylated) | ELISA | Human antibody |
High-mannose Glycoform
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-155LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Humanized antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-171LC | Recombinant Anti-Human MS4A1 Antibody (Non-glycosylated) | ELISA | Chimeric antibody (mouse/human) |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC066 | PE-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM | |
| MHC-LC067 | APC-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM | |
| MHC-LC068 | BV421-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM | |
| MHC-LC4347 | PE-B*07:02/Human MS4A1 (RPKSNIVLL) MHC Tetramer | FCM |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1751CQ | Mouse Anti-MS4A1 Recombinant Antibody (clone CBLS-211) | Neut, FuncS | Mouse IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2295 | Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone DS2295AB) | IHC-Fr, IHC-P | Rabbit IgG |
| MOR-4024 | Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone SI88DS) | FC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0144-CN | Human Anti-MS4A1 Recombinant Antibody (clone C2B8) | WB, ELISA | Chimeric (mouse/human) IgG1 |
| HPAB-0018-FY | Human Anti-MS4A1 Recombinant Antibody (HPAB-0018-FY) | FC | Humanized IgG1 |
| HPAB-0021-FY | Human Anti-MS4A1 Recombinant Antibody (clone hu3D9) | FC | Humanized IgG |
| HPAB-0819-CN | Human Anti-MS4A1 Recombinant Antibody (clone 1F5RKF12) | ELISA, FC | Humanized IgG |
| HPAB-0820-CN | Human Anti-MS4A1 Recombinant Antibody (clone RHAxRKF12) | ELISA, FC | Humanized IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0452-YC-S(P) | Canine Anti-MS4A1 Recombinant Antibody; scFv Fragment (HPAB-M0452-YC-S(P)) | FC, IHC | Canine scFv |
| HPAB-M0453-YC-S(P) | Human Anti-MS4A1 Recombinant Antibody; scFv Fragment (HPAB-M0453-YC-S(P)) | FC, IHC | Humanized scFv |
| HPAB-0346-WJ-S(P) | Human Anti-MS4A1 Recombinant Antibody (clone 7F2); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0347-WJ-S(P) | Human Anti-MS4A1 Recombinant Antibody (clone 8G3); scFv Fragment | ELISA | Humanized scFv |
| HPAB-1885-FY-F(E) | Human Anti-MS4A1 Recombinant Antibody; Fab Fragment (HPAB-1885-FY-F(E)) | ELISA, FC | Human Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-772 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ocrelizumab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| AFC-TAB-207 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ublituximab), ADCC Enhanced | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| AFC-TAB-771 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ocaratuzumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-H39 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Ibritumomab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-H51 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody (Obinutuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ACFP-SH134. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.