Anti-Human CD276 Recombinant Antibody (MGA271) (CAT#: TAB-117CL)
Recombinant Humanized monoclonal antibody expressed in CHO binding to Human CD276. MGA271 is a first-in-class, humanized IgG1κ monoclonal antibody that recognizes human B7-H3, a novel member of the B7 family of immune regulators. MGA271 has been Fc-engineered to further augment its tumor killing activity.
Specific Inquiry
ADCC
Figure 1 MGA271 mediates in vitro ADCC on A498 renal cell carcinoma cells with human PBMC effector cells representing all 3 CD16A-158 genotypes and with cynomolgus monkey PBMC effector cells.
Evaluation of the ability of MGA271 to mediate ADCC on the A498 renal cell carcinoma cell line using resting PBMCs representative of each CD16A-158 genotype--A, F/V; B, F/F; or C, V/V and compared with the indicated control molecules: humanized BRCA84D (RES240), chimeric BRCA84D (chBRCA84D), and aglycosylated humanized BRCA84D (RES240-aglycosyl). D, evaluation of the ability of cynomolgus monkey PBMCs to support MGA271-mediated ADCC on the A498 renal cell carcinoma cell line
Loo, D., Alderson, R. F., Chen, F. Z., Huang, L., Zhang, W., Gorlatov, S.,... & Son, T. (2012). Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity. Clinical cancer research, 18(14), 3834-3845.
Figure 2 MGA271 mediates in vitro ADCC across a panel of cancer cell lines exhibiting a range of B7-H3 expression.
The ability of MGA271 to mediate ADCC was evaluated on B7-H3-positive cancer cell lines with resting PBMCs. LnCAP prostate adenocarcinoma.
Loo, D., Alderson, R. F., Chen, F. Z., Huang, L., Zhang, W., Gorlatov, S.,... & Son, T. (2012). Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity. Clinical cancer research, 18(14), 3834-3845.
FuncS
Figure 3 MGA271 exhibits potent in vivo antitumor activity toward tumor cell carcinoma xenografts.
Xenografts were established by implanting A498 renal cell carcinoma, 786-0 renal cell carcinoma, or HT-1197 bladder carcinoma cells subcutaneously into the mCD16-/-hCD16A+ mice. MGA271, the indicated control mAbs, or PBS vehicle were administered by intravenous injection once weekly (arrows) at the indicated dose levels beginning 6 to 12 days (early treatment mode; A-D) or 21 days (late treatment mode, average tumor volume 260 mm³; E) following tumor cell implantation. Asterisks indicate time point at which the tumor volume for the treatment group reached statistical significance (P < 0.05) compared with IgG treatment group.
Loo, D., Alderson, R. F., Chen, F. Z., Huang, L., Zhang, W., Gorlatov, S.,... & Son, T. (2012). Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity. Clinical cancer research, 18(14), 3834-3845.
Specifications
- Derivation
- Humanized
- Type
- Antibody
- Species Reactivity
- Human
- Applications
- ADCC, FuncS
- Related Disease
- Solid Tumors
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Applications
- Application Notes
- This antibody has been tested for use in Antibody-dependent Cellular Cytotoxicity Assay and Functional Assay.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD276"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1603MZ | Mouse Anti-CD276 Recombinant Antibody (clone NJI53) | FC | Mouse IgG1 |
| HPAB-0234-CN | Human Anti-CD276 Recombinant Antibody (HPAB-0234-CN) | ELISA, FC, IF | Human IgG |
| HPAB-0241-CN | Human Anti-CD276 Recombinant Antibody (HPAB-0241-CN) | ELISA, FC, IP | Chimeric (mouse/human) IgG1, κ |
| HPAB-0048LY | Mouse Anti-CD276 Recombinant Antibody (HPAB-0048LY) | FuncS, ELISA, FC, IHC | Mouse IgG |
| HPAB-0049LY | Human Anti-CD276 Recombinant Antibody (HPAB-0049LY) | FuncS, ELISA, FC, IHC | Humanized IgG |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-333CQ | Rat Anti-CD276 Recombinant Antibody (clone MJ18) | Block, FC | Rat IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0549 | Hi-Affi™ Rabbit Anti-CD276 Recombinant Antibody (clone DS549AB) | WB, IP, FC | Rabbit IgG |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-424CQ | Anti-Human CD276 Recombinant Antibody (Enoblituzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0048LY-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0048LY-S(P)) | FuncS, ELISA, FC, IHC | Mouse scFv |
| HPAB-0049LY-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0049LY-S(P)) | FuncS, ELISA, FC, IHC | Human scFv |
| HPAB-M0422-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0422-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-M0423-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0423-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-0373-FY-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0373-FY-S(P)) | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0048LY-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0048LY-F(E)) | FuncS, ELISA, FC, IHC | Mouse Fab |
| HPAB-0049LY-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0049LY-F(E)) | FuncS, ELISA, FC, IHC | Humanized Fab |
| HPAB-M0422-YC-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0422-YC-F(E)) | Cyt, FuncS | Mouse Fab |
| HPAB-M0423-YC-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0423-YC-F(E)) | Cyt, FuncS | Mouse Fab |
| HPAB-M0424-YC-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0424-YC-F(E)) | Cyt, FuncS, IHC | Humanized Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-424CQ | Afuco™ Anti-CD276 ADCC Recombinant Antibody (Enoblituzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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