Recombinant Humanized monoclonal antibody expressed in CHO binding to Human CD98. This is a therapeutic anti-CD98 antibody with multiple mechanisms of action demonstrates anti-tumor efficacy.
Figure 1 Tumor growth inhibition of IGN523 in different tumor models.
(a) HL-60, (b) OCI-AML-3, (c) KG-1, (d) IGN-LNG-12 and (e) IGN-LNG-54 growth curves in either NOD/SCID-(c-e) or CB.17-SCID-mice (a and b) receiving IGN523 (15 mg/kg) or control IgG (15 mg/kg). Carboplatin was administered in IGN-LNG-12 and IGN-LNG-54 at 10 mg/kg i.p. (n = 8).
Hayes, G. M., Chinn, L., Cantor, J. M., Cairns, B., Levashova, Z., Tran, H.,... & Ginsberg, M. H. (2015). Antitumor activity of an anti‐CD 98 antibody. International journal of cancer, 137(3), 710-720.
Figure 2 Tumor growth inhibition of IGN523 in different tumor models.
anti-CD98 staining of representative IGN-LNG-54 tumors post-treatment. *p < 0.05; ***p< 0.001 relative to control. Starting tumor volumes were 134 ± 32 mm³ (HL-60); 174 ± 34 mm³ (OCI-AML-3); 92 ± 11 mm³ (KG-1); 126 ± 23 mm³ (IGN-LNG-12) and 136 ± 38 mm³ (IGN-LNG-54). Please note a statistical difference between IGN523 and carboplatin treatment groups in IGN-LNG-54 (p < 0.04). Arrow 5 administration of antibody. Scale bar = 20 μm.
Hayes, G. M., Chinn, L., Cantor, J. M., Cairns, B., Levashova, Z., Tran, H.,... & Ginsberg, M. H. (2015). Antitumor activity of an anti‐CD 98 antibody. International journal of cancer, 137(3), 710-720.
Figure 3 IGN523 demonstrates strong ADCC-, but not CDC-activity.
Antibody-induced luciferase activity in the effector cell is quantified with luminescence readout and is a surrogate for effector cell-mediated cell lysis. Target cells (a and d) ramos, (b and e) KG-1 and (c) OCI-AML-3 were used in (a-c) ADCC or (d and e) CDC assays. Please note that IGN523 elicits greater ADCC activity than rituximab in ramos cells. Cetuximab (anti-EGFR) was used as negative control antibody (control IgG). Ramos, KG-1 and OCI-AML-3 cell lines both endogenously express CD98 on the cell surface.
Hayes, G. M., Chinn, L., Cantor, J. M., Cairns, B., Levashova, Z., Tran, H.,... & Ginsberg, M. H. (2015). Antitumor activity of an anti‐CD 98 antibody. International journal of cancer, 137(3), 710-720.
Figure 4 IGN523 decreases viability, amino acid transport and increases caspase-3 and caspase-7 activity.
Cells were incubated with IGN523 in the presence or absence of AbXL. (a-c) cell viability was assessed by measurement of live-cell protease activity that is restricted to intact viable cells (fluorescence). (d-f) Caspase-3 and caspase-7 activity was measured by increase in aminoluciferin-induced luminescence through DEVD-aminoluciferin substrate cleavage. (g-i) crosslinked IGN523 reduced ³Phe-uptake. BCH was used at 10 mM. The ratio of IGN523:AbXL was 1:5. The amount of ³Phe-uptake in ramos cells under each treatment determined as counts per minute (cpm) is graphed. *p < 0.05; ***p < 0.001 relative to IGN523 (a and b) or control (c), respectively.
Hayes, G. M., Chinn, L., Cantor, J. M., Cairns, B., Levashova, Z., Tran, H.,... & Ginsberg, M. H. (2015). Antitumor activity of an anti‐CD 98 antibody. International journal of cancer, 137(3), 710-720.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-381LC | Anti-Human CD98 Recombinant Antibody (cM23) | FC | Chimeric antibody (mouse/human) |
TAB-396LC | Human Anti-CD98 Recombinant Antibody (TAB-396LC) | FC | Chimeric (Rat/Human) antibody |
TAB-397LC | Human Anti-CD98 Recombinant Antibody (TAB-397LC) | FC | Chimeric (Rat/Human) antibody |
TAB-396LC-F(E) | Human Anti-CD98 Recombinant Antibody; Fab Fragment (TAB-396LC-F(E)) | FC | Chimeric (Rat/Human) Fab |
TAB-397LC-F(E) | Human Anti-CD98 Recombinant Antibody; Fab Fragment (TAB-397LC-F(E)) | FC | Chimeric (Rat/Human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-385LC | Human Anti-CD98 Recombinant Antibody (TAB-385LC) | FC | Human IgG, κ |
TAB-386LC | Human Anti-CD98 Recombinant Antibody (TAB-386LC) | FC | Human IgG, κ |
TAB-387LC | Human Anti-CD98 Recombinant Antibody (TAB-387LC) | FC | Human IgG, κ |
TAB-388LC | Human Anti-CD98 Recombinant Antibody (TAB-388LC) | FC | Human IgG, κ |
TAB-389LC | Human Anti-CD98 Recombinant Antibody (TAB-389LC) | FC, FuncS | Human IgG, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-382LC-S(P) | Anti-Human CD98 Recombinant Antibody scFv Fragment (hM23-H1L1) | FC | Humanized antibody |
TAB-383LC-S(P) | Anti-Human CD98 Recombinant Antibody scFv Fragment (hM23-H1L2) | FC | Humanized antibody |
TAB-384LC-S(P) | Anti-Human CD98 Recombinant Antibody scFv Fragment (hM23-H2L2) | FC | Humanized antibody |
TAB-382LC-F(E) | Anti-Human CD98 Recombinant Antibody Fab Fragment (hM23-H1L1) | FC | Humanized antibody |
TAB-383LC-F(E) | Anti-Human CD98 Recombinant Antibody Fab Fragment (hM23-H1L2) | FC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0617-YJ | Human Anti-CD98 Recombinant Antibody (clone CP3-10) | ELISA, IF, IP, Inhib, FuncS | Mouse IgG |
HPAB-0618-YJ | Human Anti-CD98 Recombinant Antibody (clone chCP3-10) | ELISA, IF, IP, Inhib, FuncS | Chimeric (mouse/human) IgG1 |
FAMAB-1568CQ | Human Anti-CD98 Recombinant Antibody (clone CP1-5) | ELISA | Human IgG1 |
FAMAB-1571CQ | Mouse Anti-CD98 Recombinant Antibody (clone RL388) | IP, Stim, FC | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0617-YJ-F(E) | Human Anti-CD98 Recombinant Antibody (clone CP3-10); Fab Fragment | ELISA, IF, IP, Inhib, FuncS | Human Fab |
HPAB-0618-YJ-F(E) | Human Anti-CD98 Recombinant Antibody (clone chCP3-10); Fab Fragment | ELISA, IF, IP, Inhib, FuncS | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0617-YJ-S(P) | Human Anti-CD98 Recombinant Antibody (clone CP3-10); scFv Fragment | ELISA, IF, IP, Inhib, FuncS | Human scFv |
HPAB-0618-YJ-S(P) | Mouse Anti-CD98 Recombinant Antibody (clone chCP3-10); scFv Fragment | ELISA, IF, IP, Inhib, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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