Anti-DENV2 E Recombinant Antibody (MRO-1132LC) (CAT#: MRO-1132LC)
This product is a recombinant mouse anti-Dengue virus-2 monoclonal antibody. This antibody specifically binds to the domain III of dengue virus envelope glycoprotein. This monoclonal antibody is capable of specifically binding and neutralizing dengue virus. This antibody product can be potentially used in the research of Dengue virus-2 infection.
Specific Inquiry
Neut
Figure 1 Virus neutralization and mode of action of 9F12.
Pre- and post-adsorption assay. Pre-incubated mixtures of DENV-2 with mAb 9F12 (open squares) or 4G2 (open circles) were dispensed onto Vero cell monolayers at 4 °C for 1 h in the pre-adsorption assay. The virus antibody mixture was replaced with diluted virus in the post-adsorption assay, followed by washing and addition of either mAb 9F12 (filled squares) or 4G2 (filled circles).
On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
ELISA
Figure 2 Binding of DENV-1–4 and WNV to 9F12.
The assay was carried out in triplicate and the values displayed represent the average. Error bars, sd.
On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
FC
Figure 3 Epitope mapping.
Flow cytometry histograms of mAbs binding to yeast expressing wild-type or mutant domain III. The following antibodies were used as negative and positive staining controls, respectively, for each of the indicated yeast based on data from a prior publication: wild-type, WNV E16 and 3H5-1; K305E, 1A1D-2 and 3H5-1; K307C, 1A1D-2 and 3H5-1; K310E, 1A1D-2 and 3H5-1; P384A, 3H5-1 and 5A2-7; G330D, 6B6-10 and 3H5-1. In each case, staining with 9F12 is shown in red. The data are representative of three independent experiments.
On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes
ELISA
Figure 4 ELISA results of the antibody.
Elisa for C9 IgG (30 nmol/L) against DENV2 whole virus particles competed with increasing concentrations of mouse 9F12 and 3H5 IgG. C9 IgG binding was detected with an anti-human horseradish peroxidase (HRP) conjugate.
Phage Display Approaches for the Isolation of Monoclonal Antibodies Against Dengue Virus Envelope Domain III from Human and Mouse Derived Libraries
Specifications
- Host Species
- Mouse
- Type
- Mouse Antibody
- Specificity
- Dengue virus-2
- Clone
- 9F12
- Applications
- ELISA, Neut, FC
- Related Disease
- Dengue virus-2 infection
Applications
- Application Notes
- The DENV2 E antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization, Flow Cytometry.
ELISA: Microtitre plates coated with recombinant domain III (0.2 μg per well) in a carbonate-bicarbonate buffer were incubated with tenfold dilutions of mAb 9F12 for 3 h at 22 °C. After washing, the amount of mAb 9F12 binding to the plate was detected using an anti-mouse antibody conjugated with alkaline phosphatase for 1 h at 22 °C, followed by addition of p-nitrophenyl phosphate (Pierce), and the optical density was measured at 405 nm (OD405).
Target
- Alternative Names
- Dengue virus-2; DENV2; Dengue virus-2 envelope protein E; DENV2 E
- Gene ID
- 1494449
- UniProt ID
- A0A173DS53
- Long Name
- Dengue virus-2 envelope protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 9F12"
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Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1106LC | Mouse Anti-DENV2 E Recombinant Antibody (MRO-1106LC) | ELISA, WB, Neut | Mouse IgG1, κ |
| MRO-1107LC | Mouse Anti-DENV2 E Recombinant Antibody (MRO-1107LC) | WB, ELISA | Mouse IgG1, κ |
| MRO-1110LC | Mouse Anti-DENV2 E Recombinant Antibody (MRO-1110LC) | WB, ELISA | Mouse IgG1, κ |
| MRO-1106LC-F(E) | Mouse Anti-DENV2 E Recombinant Antibody; Fab Fragment (MRO-1106LC-F(E)) | ELISA, WB | Mouse Fab |
| MRO-1107LC-F(E) | Mouse Anti-DENV2 E Recombinant Antibody; Fab Fragment (MRO-1107LC-F(E)) | ELISA, WB | Mouse Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1114LC | Human Anti-DENV2 E Recombinant Antibody (MRO-1114LC) | ELISA | Human IgG |
| MRO-1115LC | Human Anti-DENV2 E Recombinant Antibody (MRO-1115LC) | ELISA | Human IgG |
| MRO-1116LC | Human Anti-DENV2 E Recombinant Antibody (MRO-1116LC) | ELISA | Human IgG |
| MRO-1117LC | Human Anti-DENV2 E Recombinant Antibody (MRO-1117LC) | ELISA | Human IgG |
| MRO-1119LC | Human Anti-DENV2 E Recombinant Antibody (MRO-1119LC) | ELISA | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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