Recombinant Human Antibody (Apomab) is capable of binding to DR5, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-DR5 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-DR5 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody induces tumor cell apoptosis through DR5 and investigated the structural features of its interaction with DR5.
Figure 1 ELISA analysis of Apomab binding to DR5.
Apomab binding to purified human IgG1 Fc fusion proteins based on the human Apo2L/TRAIL receptors DR5, DR4, DcR1, DcR2 and OPG (Genentech Inc.). Binding was determined in ELISA format: each receptor–Fc fusion was coated onto microtiter wells and incubated with a serial dilution of Apomab for 2 h at room temperature. Bound Apomab was then detected using a horseradish peroxidase-conjugated goat F(ab′)2 anti-human IgG antibody.
Adams, C., Totpal, K., Lawrence, D., Marsters, S., Pitti, R., Yee, S., ... & Compaan, D. (2008). Structural and functional analysis of the interaction between the agonistic monoclonal antibody Apomab and the proapoptotic receptor DR5. Cell death and differentiation, 15(4), 751.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0245MC | Human Anti-DR5 Recombinant Antibody (clone YC037) | ELISA, WB, IHC, FuncS | |
HPAB-0621LY | Mouse Anti-DR5 Recombinant Antibody (HPAB-0621LY) | FC | Mouse IgG |
HPAB-0622LY | Mouse Anti-DR5 Recombinant Antibody (HPAB-0622LY) | FC | Mouse IgG |
HPAB-0728LY | Human Anti-DR5 Recombinant Antibody (clone mAb1) | ELISA | Human IgG |
HPAB-0729LY | Human Anti-DR5 Recombinant Antibody (clone mAb2) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-450 | Human Anti-DR5 Recombinant Antibody (clone Apomab); Fab Fragment | FC, ELISA | Human Fab |
HPAB-0621LY-F(E) | Mouse Anti-DR5 Recombinant Antibody; Fab Fragment (HPAB-0621LY-F(E)) | FC | Mouse Fab |
HPAB-0622LY-F(E) | Mouse Anti-DR5 Recombinant Antibody; Fab Fragment (HPAB-0622LY-F(E)) | FC | Mouse Fab |
HPAB-0728LY-F(E) | Human Anti-DR5 Recombinant Antibody (clone mAb1); Fab Fragment | ELISA | Human Fab |
HPAB-0729LY-F(E) | Human Anti-DR5 Recombinant Antibody (clone mAb2); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-450 | Human Anti-DR5 Recombinant Antibody (clone Apomab); scFv Fragment | FC, ELISA | Human scFv |
HPAB-0621LY-S(P) | Mouse Anti-DR5 Recombinant Antibody; scFv Fragment (HPAB-0621LY-S(P)) | FC | Mouse scFv |
HPAB-0622LY-S(P) | Mouse Anti-DR5 Recombinant Antibody; scFv Fragment (HPAB-0622LY-S(P)) | FC | Mouse scFv |
HPAB-0728LY-S(P) | Human Anti-DR5 Recombinant Antibody (clone mAb1); scFv Fragment | ELISA | Human scFv |
HPAB-0729LY-S(P) | Human Anti-DR5 Recombinant Antibody (clone mAb2); scFv Fragment | ELISA | Human scFv |
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