Mouse Anti-SELE Recombinant Antibody (clone 7A9) (CAT#: PABW-041)

Figure 1 FACS analysis of the intact 7A9 antibody and the F(ab9)2 fragment was performed in the presence (+) and absence (-) of IL-1b (1 mg/ml).

The analysis was performed on a Coulter (Epex) FACS analyzer.

Rodrıguez-Romero, A., Almog, O., Tordova, M., Randhawa, Z., & Gilliland, G. L. (1998). Primary and tertiary structures of the Fab fragment of a monoclonal anti-E-selectin 7A9 antibody that inhibits neutrophil attachment to endothelial cells. Journal of Biological Chemistry, 273(19), 11770-11775.

Figure 2 Effect of 7A9 F(ab‘)2 on granulocyte adhesion of HL60.

The confluent HL60 in 16-mm wells were left untreated (-) or treated (+) with 1 mg/ml IL-1b for 4 h. After rinsing to remove IL-1b, cells were incubated with F(ab')2 at the indicated concentration for 30 min at 37 °C. Results are expressed as cells bound/well.

Rodrıguez-Romero, A., Almog, O., Tordova, M., Randhawa, Z., & Gilliland, G. L. (1998). Primary and tertiary structures of the Fab fragment of a monoclonal anti-E-selectin 7A9 antibody that inhibits neutrophil attachment to endothelial cells. Journal of Biological Chemistry, 273(19), 11770-11775.

Figure 3 Endostatin does not block E-selectin-mediated leukocyte adhesion.

HL-60 cell adhesion to HDMEC treated for 4 h with LPS (0.1 μg/ml) was measured in the presence of 0-10 μg/ml endostatin (ES), EDTA (2.5 mM), neutralizing antibody (7A9) to E-selectin (1 μg/ml), or isotype-matched IgG1 (1 μg/ml). Values shown are the total bound cells counted in 10 randomly selected microscopic fields by using a 20× objective.

Yu, Y., Moulton, K. S., Khan, M. K., Vineberg, S., Boye, E., Davis, V. M., ... & Milstone, D. S. (2004). E-selectin is required for the antiangiogenic activity of endostatin. Proceedings of the National Academy of Sciences, 101(21), 8005-8010.

Figure 4 Endostatin does not block E-selectin-mediated leukocyte adhesion.

Images of HL-60 (round cells) on HUVEC in the absence (a) or presence of 10 μg/ml endostatin (b), 1 μg/ml mIgG1 (c), or 1 μg/ml 7A9 (d).

Yu, Y., Moulton, K. S., Khan, M. K., Vineberg, S., Boye, E., Davis, V. M., ... & Milstone, D. S. (2004). E-selectin is required for the antiangiogenic activity of endostatin. Proceedings of the National Academy of Sciences, 101(21), 8005-8010.