Human Anti-ECD Recombinant Antibody (clone mAb23) (CAT#: PABL-460)
Recombinant Human Antibody (mAb23) is capable of binding to ECD, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ECD mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ECD proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody blocks glucagon binding and inhibits basal receptor activity, indicating that it is an inverse agonist and that the ECD can negatively regulate receptor activity independent of ligand binding.
Specific Inquiry
Figure 1 Antibody mAb1 blocks glucagon-induced PEPCK gene expression in human hepatocytes.
Average IC50s (nM) from two experiments are 0.15, 1.5, and 0.4 for mAb1, mAb7, and mAb23, respectively.
Koth, C. M., Murray, J. M., Mukund, S., Madjidi, A., Minn, A., Clarke, H. J., ... & Rondon, J. (2012). Molecular basis for negative regulation of the glucagon receptor. Proceedings of the National Academy of Sciences, 109(36), 14393-14398.
Specifications
- Immunogen
- Human ecdysoneless homolog (Drosophila)
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human ECD
- Species Reactivity
- Human
- Clone
- mAb23
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ECD; ecdysoneless homolog (Drosophila); GCR2; HSGT1; protein SGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2;
- Gene ID
- 11319
- UniProt ID
- O95905
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-459 | Human Anti-ECD Recombinant Antibody (clone mAb1) | ELISA, WB, FuncS | Human IgG |
| MOB-1843MZ | Recombinant Mouse Anti-Human ECD Antibody | ELISA, WB | Mouse antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-456 | Human Anti-ECD Recombinant Antibody (clone mAb1); Fab Fragment | ELISA, WB, FuncS | Human Fab |
| PFBL-457 | Human Anti-ECD Recombinant Antibody (clone mAb23); Fab Fragment | ELISA, WB, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-456 | Human Anti-ECD Recombinant Antibody (clone mAb1); scFv Fragment | ELISA, WB, FuncS | Human scFv |
| PSBL-457 | Human Anti-ECD Recombinant Antibody (clone mAb23); scFv Fragment | ELISA, WB, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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