Mouse Anti-FCGR2B Recombinant Antibody (TAB-036WM) (CAT#: TAB-036WM)

This product is a recombinant mouse antibody that recognizes Fc-gamma-R2B (Fc fragment of IgG receptor IIb). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.


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  • Datasheet
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  • COA
WB

Figure 1 Mouse Anti-FCGR2B Antibody (TAB-036WM) in WB

Figure 1 Mouse Anti-FCGR2B Antibody (TAB-036WM) in WB

Western blot analysis of TAB-036WM was performed by loading Human FCGR2B Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-036WM (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

DB

Figure 2 Mouse Anti-Human FCGR2B Antibody (TAB-036WM) in DB

Figure 2 Mouse Anti-Human FCGR2B Antibody (TAB-036WM) in DB

Dot Blot analysis of TAB-036WM was performed by coating with Human FCGR2B Protein (His Tag).

TAB-036WM incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)

ELISA

Figure 3 Mouse Anti-Human FCGR2B Antibody (TAB-036WM) in ELISA

Figure 3 Mouse Anti-Human FCGR2B Antibody (TAB-036WM) in ELISA

ELISA analysis of TAB-036WM was performed by coating with Human FCGR2B Protein (His Tag). Then blocked with BSA and incubated with TAB-036WM. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human Fc-gamma-R2B (Fc fragment of IgG receptor IIb)
  • Species Reactivity
  • Human
  • Applications
  • ELISA, FC, Inhib
  • Related Disease
  • Human Primary Ovarian, Breast Carcinoma

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • See the COA
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • FCGR2B; Fc fragment of IgG, low affinity IIb, receptor (CD32); CD32; FCG2; CD32B; FCGR2; IGFR2; low affinity immunoglobulin gamma Fc region receptor II-b; CDw32; fcRII-b; Fc gamma RIIb; fc-gamma-RIIb; fc-gamma RII-b; igG Fc receptor II-b; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32)

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "FCGR2B"

Rat Antibody

CAT Product Name Application Type
TAB-267CL Human Anti-FCGR2B Recombinant Antibody (TAB-267CL) FC, WB Human IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-037WM Human Anti-FCGR2B Recombinant Antibody (TAB-037WM) ELISA, FuncS Chimeric (human/mouse) IgG
TAB-037WM-S(P) Anti-FCGR2B Recombinant Antibody; scFv Fragment (TAB-037WM-S(P)) ELISA, FC, WB Mouse scFv
TAB-037WM-F(E) Human Anti-FCGR2B Recombinant Antibody; Fab Fragment (TAB-037WM-F(E)) ELISA, FC, WB Chimeric Antibody (human/mouse)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1273 Hi-Affi™ Rabbit Anti-FCGR2B Recombinant Antibody (clone DS1273AB) WB, IHC, IP, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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