Recombinant Human Antibody (HK20) is capable of binding to HIV-1 gp41, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp41 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp41 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. HK20 IgG have limited neutralization breadth and potency while smaller HK20 Fabs and scFv reveal a broad cross clade neutralization breadth.
Figure 1 Immunoprecipitation of HR-1, 5HB and HR-1-FP constructs in the presence (+) or absence (-) of HK20 mAb.
Proteins were separated in 10% polyacrylamide gels under reducing conditions and stained with Coomassie blue. C, HK20 mAb alone; MW, molecular weight.
CORTI, Davide, et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PloS one, 2010, 5.1.
Figure 2 HK20 binds to the HR-1 region within gp41.
HK20 binding to gp41 constructs by ELISA.
CORTI, Davide, et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PloS one, 2010, 5.1.
Figure 3 HK20 binds to the HR-1 region within gp41.
Neutralization of CH064.20 HIV-1 primary isolates by HK20 IgG and Fab fragments in a HOS-based assay.
CORTI, Davide, et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PloS one, 2010, 5.1.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-486-F(E) | Recombinant Anti-HIV-1 gp41 Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
PFBL-185 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 8062); Fab Fragment | WB, ELISA, FuncS | Human Fab |
PFBL-186 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 2F5); Fab Fragment | WB, Neut, FuncS | Human Fab |
PFBL-187 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC176); Fab Fragment | Neut, FuncS | Human Fab |
PFBL-188 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC315); Fab Fragment | Neut, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-486-S(P) | Recombinant Human Anti-HIV-1 gp41 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
PSBC-331 | Mouse Anti-HIV-1 gp41 Recombinant Antibody; scFv Fragment (PSBC-331) | Neut | Mouse scFv |
PSBC-332 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 10E8v4-5R+100cF); scFv Fragment | Neut | Human scFv |
PSBC-334 | Mouse Anti-HIV-1 gp41 Recombinant Antibody; scFv Fragment (PSBC-334) | ELISA, Neut | Mouse scFv |
PSBC-335 | Mouse Anti-HIV-1 gp41 Recombinant Antibody; scFv Fragment (PSBC-335) | ELISA, Neut | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABZ-068 | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 1583) | Neut, FC | Mouse IgG2a, κ |
MRO-842CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 1911) | WB | Mouse IgG2a |
MRO-843CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 191) | WB | Mouse IgG2 |
MRO-844CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 10E9) | WB, RIPA, ELISA, IF | Mouse IgG1 |
MRO-845CQ | Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone 10F6) | ELISA, WB, IP, IF | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-074LC | Anti-HIV-1 gp41 Recombinant Antibody (W) | ELISA | Human antibody |
MRO-077LC | Anti-HIV-1 gp41 Recombinant Antibody (3D6) | ELISA | Human antibody |
MRO-079LC | Anti-HIV-1 gp41 Recombinant Antibody (3D9) | ELISA | Human antibody |
MRO-093LC | Anti-HIV-1 gp41 Recombinant Antibody (clone 43) | ELISA | Human antibody |
MRO-025LC-F(E) | Anti-HIV-1 gp41 Recombinant Antibody Fab Fragment (4E10-IgG3) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC746 | A*2402/HIV-1 gp41 (RYLKDQQLL) MHC Tetramer | FCM |
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