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Mouse Anti-CASP4 Recombinant Antibody (clone DBT5) (CAT#: MOB-0327CT)

Recombinant Mouse monoclonal antibody to Human CASP4, expressed in mammalian cells.


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Specific Inquiry
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  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/if_selected.jpg
Figure 2 IHC staining of human duodenum
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/ihc_selected.jpg
Figure 3 IHC staining of human stomach
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows membrane and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37167/ihc_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/321_G12_5_blue_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/323_G12_3_blue_red_green.jpg
Normal Tissue
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_B_9_5.jpg
Figure 7 Lung
Figure 7 Lung
(Macrophages
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_2_4.jpg
Figure 8 Colon
Figure 8 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_9_3.jpg
Figure 9 Liver
Figure 9 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_7_4.jpg
Figure 10 Testis
Figure 10 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_4_6.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27588/71605_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196954-CASP4

Specifications

  • Immunogen
  • Synthetic peptide: KERAEEIYPIKERNN conjugated to KLH, corresponding to amino acids 119-133 of Human Caspase 4.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Species Reactivity
  • Human
  • Clone
  • DBT5
  • Applications
  • ELISA, WB
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • 2.2 mg/ml
  • Buffer
  • Preservative: 15mM Sodium Azide Constituents: 0.01M PBS, pH 7.4
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • CASP4; caspase 4, apoptosis-related cysteine peptidase; TX; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II; caspase-4; CASP-4; ICE(rel)-II; protease TX; protease ICH-2; apoptotic cysteine protease Mih1/TX; caspase 4, apoptosis-related cysteine protease;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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