Mouse Anti-KDO Recombinant Antibody (clone S54-10) (CAT#: PABW-155)
Recombinant Mouse Antibody (S54-10) is capable of binding to Kdo. MAb S54-10 bound to the C. psittaci specific structures to a similar extent as S73-2 but exhibited lower affinity for all other antigens tested. S54-10 did not differentiate between antigens containing the branched Kdo tetrasaccharide or the (2→4)-(2→4)-linked trisaccharide, but did bind to the 2→8-2→4-linked trisaccharide with weaker affinity than S73-2. S54-10 bound with very high affinity to Kdo(2→4)Kdo(2→4) and bound o nly weakly to the Kdo(2→8)Kdo(2→4) trisaccharide. Thus S54-10 is highly specific for the Kdo(2→4)Kdo(2→4) trisaccharide.
Specific Inquiry
SPR
Figure 1 Surface plasmon resonance analysis of S54-10 Fab binding to different Kdo antigens
Sensorgram overlays for the binding of 5, 10, 20, 40, 80 and 160 nM S54-10 Fab to 280 RUs of immobilized Kdo(2→4)Kdo(2→4)Kdo-BSA. The open black circles are the data points and the red curves the fitting of the data to a 1:1 interaction model.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
SPR
Figure 2 Surface plasmon resonance analysis of S54-10 Fab binding to different Kdo antigens
Sensorgram overlays for the binding of 2.5, 5, 7.5, 10, 15 and 20 nM S54-10 Fab binding to immobilized Kdo(2→4)Kdo(2→4)Kdo-BSA.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
SPR
Figure 3 Surface plasmon resonance analysis of S54-10 Fab binding to different Kdo antigens
Standard curves for S54-10 Fab concentration assay based on initial mass transport limited rates from the sensorgrams in D - the inset shows linear fitting (red lines) of the initial data points (open black circles).
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
SPR
Figure 4 Surface plasmon resonance analysis of S54-10 Fab binding to different Kdo antigens
Fitting to a solution affinity model of data for the inhibition of S54-10 Fab binding to 280 RUs of immobilized Kdo(2→4)Kdo(2→4)Kdo-BSA by various saccharides (○)Kdo; Kdo(2→8)Kdo; (◆) Kdo(2→8)Kdo(▼)Kdo(2→4)Kdo; (□)Kdo(2→8)Kdo(2→4)Kdo; (■)Kdo(2→4)Kdo(2→4)Kdo.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
Specifications
- Immunogen
- 3-deoxy-α-D-manno-oct-2-ulosonic acid
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Tested positive against synthetic Kdo (3-deoxy-α-D-manno-oct-2-ulosonic acid)
- Clone
- S54-10
- Applications
- ELISA
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
- Alternative Names
- Kdo; 3-Deoxy-D-manno-oct-2-ulosonic acid; 2-Oxo-3-deoxy-D-mannooctonic acid; 2-Keto-3-Deoxy-D-manno-octonate; 2-Keto-3-deoxy-D-mannooctanoic acid; 3-Deoxy-D-manno-octulosonic acid; 3-Deoxy-D-manno-2-octulosonic acid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-598 | Recombinant Anti-KDO Antibody | ELISA, WB, Neut, FuncS | IgG |
| MHH-598 | Recombinant Human Anti-KDO Antibody | ELISA, WB, IP, FuncS | IgG |
| PABL-254 | Recombinant Mouse Anti-KDO Antibody (S25-2 ) | WB, IHC, FuncS | IgG |
| PABL-626 | Mouse Anti-KDO Recombinant Antibody (clone S25-39) | ELISA | Mouse IgG |
| PABC-237 | Mouse Anti-KDO Recombinant Antibody (clone S25-26) | ELISA | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-598-F(E) | Recombinant Human Anti-KDO Antibody Fab Fragment | FC, IP, FuncS | Fab |
| PFBL-621 | Mouse Anti-KDO Recombinant Antibody (clone S25-39); Fab Fragment | ELISA | Mouse Fab |
| PFBL-622 | Mouse Anti-KDO Recombinant Antibody (clone S64-4); Fab Fragment | ELISA | Mouse Fab |
| PFBL-623 | Mouse Anti-KDO Recombinant Antibody (clone S67-27); Fab Fragment | ELISA | Mouse Fab |
| PFBL-624 | Mouse Anti-KDO Recombinant Antibody (clone S73-2); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-598-S(P) | Recombinant Human Anti-KDO Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
| PSBL-254 | Recombinant Mouse Anti-KDO Antibody scFv Fragment (S25-2 ) | WB, IHC, FuncS | scFv |
| PSBL-621 | Mouse Anti-KDO Recombinant Antibody (clone S25-39); scFv Fragment | ELISA | Mouse scFv |
| PSBL-622 | Mouse Anti-KDO Recombinant Antibody (clone S64-4); scFv Fragment | ELISA | Mouse scFv |
| PSBC-237 | Mouse Anti-KDO Recombinant Antibody (clone S25-26); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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