Recombinant Mouse Antibody (S73-2) is capable of binding to Kdo, expressed in Chinese Hamster Ovary cells (CHO). The antibody S73-2 displayed a broad specificity, binding with the same affinity to its immunizing antigen Kdo(2→8)[Kdo(2→4)]Kdo and all structures containing the 2→4-2→4-linked trisaccharide or the branched Kdo tetrasaccharide. The antibody cross-reacted with the family-specific Kdo(2→8)Kdo(2→4)Kdo linked trisaccharide and with both, the 2→4 and 2→8-linked disaccharides indicating that the antibody may not discriminate between C. psittaci and the other chlamydiae species.
Figure 1 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Sensorgram overlays for the binding of 10, 20, 40, 80, 160, 320, 640 and 1,280 nM S73-2 Fab to 160 RUs of immobilized Kdo(2→8)Kdo(2→4)Kdo-BSA. The open black circles are the data points and the red curves the fitting of the data to a 1:1 interaction model.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
Figure 2 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Sensorgram overlays for the binding of 1, 2, 3, 5, 7 and 10 nM S73-2 Fab to 2,460 RUs of immobilized Kdo(2→8)Kdo(2→4)Kdo-BSA.
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
Figure 3 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Standard curves for S73-2 Fab concentration assay based on the steady state binding data from C- the inset shows linear fitting (red lines) of the initial data points (open black circles).
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
Figure 4 Surface plasmon resonance analysis of S73-2 Fab binding to different Kdo antigens
Fitting to a solution affinity model of data for the inhibition of S73-2 Fab binding to 1,650 or 2,460 RUs of immobilized Kdo(2→8)Kdo(2→4)Kdo-BSA by various saccharides (○)Kdo; Kdo(2→8)Kdo; (◆) Kdo(2→8)Kdo(▼)Kdo(2→4)Kdo; (□)Kdo(2→8)Kdo(2→4)Kdo; (■)Kdo(2→4)Kdo(2→4)Kdo
Brooks, C. L., Müller-Loennies, S., Borisova, S. N., Brade, L., Kosma, P., Hirama, T., ... & Evans, S. V. (2010). Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry, 49(3), 570-581.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-598-S(P) | Recombinant Anti-KDO Antibody scFv Fragment | Neut, WB, FuncS | scFv |
MHH-598-S(P) | Recombinant Human Anti-KDO Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
PFBW-150 | Recombinant Mouse Anti-Kdo Antibody Fab Fragment (S67-27) | ELISA | Fab |
PFBW-155 | Mouse Anti-KDO Recombinant Antibody (clone S54-10); scFv Fragment | ELISA | Mouse Fab |
PFBW-156 | Recombinant Mouse Anti-Kdo Antibody Fab Fragment (S73-2) | ELISA | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-254 | Recombinant Mouse Anti-KDO Antibody (S25-2 ) | WB, IHC, FuncS | IgG |
PABL-626 | Mouse Anti-KDO Recombinant Antibody (clone S25-39) | ELISA | Mouse IgG |
PABL-627 | Mouse Anti-KDO Recombinant Antibody (clone S64-4) | ELISA | Mouse IgG |
PABL-628 | Mouse Anti-KDO Recombinant Antibody (clone S67-27) | ELISA | Mouse IgG |
PABL-629 | Mouse Anti-KDO Recombinant Antibody (clone S73-2) | ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-254 | Recombinant Mouse Anti-KDO Antibody Fab Fragment (S25-2 ) | WB, IHC, FuncS | Fab |
PFBL-621 | Mouse Anti-KDO Recombinant Antibody (clone S25-39); Fab Fragment | ELISA | Mouse Fab |
PFBL-622 | Mouse Anti-KDO Recombinant Antibody (clone S64-4); Fab Fragment | ELISA | Mouse Fab |
PFBL-623 | Mouse Anti-KDO Recombinant Antibody (clone S67-27); Fab Fragment | ELISA | Mouse Fab |
PFBL-624 | Mouse Anti-KDO Recombinant Antibody (clone S73-2); Fab Fragment | ELISA | Mouse Fab |
There are currently no Customer reviews or questions for PABW-156. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.