Anti-LPA Recombinant Antibody (LT3015 [B7(LT3000, Lpathomab)]) (CAT#: TAB-0340CL)
This humanized antibody LT3015 is generated from the high-affinity, specific anti-LPA mouse mAb LT3000, also called Lpathomab. The target of the antibody lysophosphatidic acid is a bioactive lipid mediating multiple cellular responses, and it's involved in the establishment and progression of cancer by providing a pro-growth tumor microenvironment and promoting angiogenesis. The EC₅₀ of the humanized antibody to 12:0 (lauroyl) LPA was determined to be 75.6 ng/mL by ELISA.
Specific Inquiry
Figure 1 LPA binding affinity measured as in Figure for native LT3015 (WT) and three LT3015 single point mutations.
Fleming, J. K., Wojciak, J. M., Campbell, M. A., & Huxford, T. (2011). Biochemical and structural characterization of lysophosphatidic Acid binding by a humanized monoclonal antibody. Journal of molecular biology, 408(3), 462-476.
Figure 2 Site-directed mutagenesis and LPA binding assays of LT3015.
In comparison to native LT3015 (WT), the introduction of mutations in the LT3015 heavy chain (AsnH52Tyr/SerH52Tyr) or light chain (AnsL30Arg) does not significantly increase LPA affinity. However, their combination (AsnH52Tyr/SerH54Tyr/AsnL30Arg) results in a modified version of the antibody with increased LPA binding affinity.
Fleming, J. K., Wojciak, J. M., Campbell, M. A., & Huxford, T. (2011). Biochemical and structural characterization of lysophosphatidic Acid binding by a humanized monoclonal antibody. Journal of molecular biology, 408(3), 462-476.
Figure 3 is a plot of data for neuron-forming neurospheres in the absence or presence of LPA (1 μM) and/or anti-LPA mAbs (1 mg/ml) for 3 days.
Specifications
- Host Species
- Human
- Type
- Humanized Antibody
- Clone
- LT3015 [B7(LT3000, Lpathomab)]
- Related Disease
- Macular degeneration, pain-related disorders, malignancies, angiogenesis, fibrosis-related disorders
Target
- Alternative Names
- LPA; Lysophosphatidic acid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-329 | Recombinant Human Anti-S. aureus LPA Antibody scFv Fragment | WB, ELISA, FuncS | scFv |
| HPAB-0253-YC-S(P) | Mouse Anti-LPA Recombinant Antibody (clone 2D1); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-0254-YC-S(P) | Mouse Anti-LPA Recombinant Antibody (clone B3); scFv Fragment | ELISA, FuncS | Mouse scFv |
| HPAB-0255-YC-S(P) | Human Anti-LPA Recombinant Antibody (clone hB3); scFv Fragment | ELISA, FuncS | Humanized scFv |
| HPAB-1295-FY-F(E) | Human Anti-LPA Recombinant Antibody (clone B7); scFv Fragment | ELISA | Humanized scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-329 | Recombinant Human Anti-S. aureus LPA Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
| HPAB-0253-YC-F(E) | Mouse Anti-LPA Recombinant Antibody (clone 2D1); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-0254-YC-F(E) | Mouse Anti-LPA Recombinant Antibody (clone B3); Fab Fragment | ELISA, FuncS | Mouse Fab |
| HPAB-0255-YC-F(E) | Human Anti-LPA Recombinant Antibody (clone hB3); Fab Fragment | ELISA, FuncS | Humanized Fab |
| HPAB-0775-WJ-F(E) | Mouse Anti-LPA Recombinant Antibody (clone B58); Fab Fragment | ELISA | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0340CL-S(P) | Anti-LPA Recombinant Antibody scFv Fragment (LT3015 [B7(LT3000, Lpathomab)]) | ELISA, SPR, Inhib, FuncS | Humanized Antibody |
| TAB-0340CL-F(E) | Anti-LPA Recombinant Antibody Fab Fragment (LT3015 [B7(LT3000, Lpathomab)]) | ELISA, SPR, Inhib, FuncS | Humanized Antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1248MZ | Recombinant Mouse Anti-Human LPA Antibody (clone 9G7B0,9I6D6) | ELISA, IHC-P, WB | Mouse antibody |
| HPAB-0253-YC | Mouse Anti-LPA Recombinant Antibody (clone 2D1) | ELISA, WB | Mouse IgG |
| HPAB-0254-YC | Mouse Anti-LPA Recombinant Antibody (clone B3) | ELISA, FuncS | Mouse IgG |
| HPAB-1295-FY | Human Anti-LPA Recombinant Antibody (clone B7) | ELISA, Inhib | Humanized IgG1, κ |
| VS3-XY1017 | Mouse Anti-LPA Recombinant Antibody (clone 4H1) | ELISA, WB, IHC, ICC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2076 | Hi-Affi™ Recombinant Rabbit Anti-LPA Monoclonal Antibody (DS2076AB) | WB, IHC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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