Mouse Anti-LPS Recombinant Antibody (clone S45-18) (CAT#: PABW-013)
Recombinant Mouse Antibody (S45-18) is capable of binding to Bacterial LPSs. The mAb S45-18 is specific for the trisaccharide αKdo(2→8)-αKdo(2→4)-αKdo trisaccharide epitope.
Specific Inquiry
Figure 1 ELISA binding of mAb.
ELISA binding of mAb S45-18 (•) and Fab S45-18 (▼), and mAb S69-4 (▲) binding to HS4P (A), 2.4/2.4PS4P (B) and 2.8/2.4PS4P ©.
Gerstenbruch, S., Brooks, C. L., Kosma, P., Brade, L., MacKenzie, C. R., Evans, S. V., ... & Müller-Loennies, S. (2009). Analysis of cross-reactive and specific anti-carbohydrate antibodies against lipopolysaccharide from Chlamydophila psittaci. Glycobiology, 20(4), 461-472.
Figure 2 SPR sensorgrams of scFv against immobilized neoglycoconjugates NH2240-31 (A and D), scFv S69-4 H3 post (B and E) and Fab S45-18 (C and F) against HS4P (A, B, C) and 2.4/2.4PS4P (D, E, and F).
Sensorgrams recorded for protein concentrations of 1 nM, 2.5 nM, 5 nM, 7.5 nM, 10 nM, 20 nM, and 40 nM are shown in A and B. Identical concentrations are displayed with same colors. Proteins were purified by gel filtration immediately prior to the analysis.
Gerstenbruch, S., Brooks, C. L., Kosma, P., Brade, L., MacKenzie, C. R., Evans, S. V., ... & Müller-Loennies, S. (2009). Analysis of cross-reactive and specific anti-carbohydrate antibodies against lipopolysaccharide from Chlamydophila psittaci. Glycobiology, 20(4), 461-472.
Figure 4 Detection of chlamydial inclusions in tissue culture with monoclonal antibodies S5-10, S69-4, Fab of S45-18 and NH2240-31.
Tissue culture monolayers were infected with C. psittaci, C. pneumoniae, or C. trachomatis, and after fixation incubated with the indicated concentrations of antibody or fragments thereof. Reactions with scFv were developed with a c-myc-specific second antibody (clone 9E10) and a third IgG H+L binding antibody conjugated to FITC. For analyses using Fab or mAb, the second antibody was omitted.
Gerstenbruch, S., Brooks, C. L., Kosma, P., Brade, L., MacKenzie, C. R., Evans, S. V., ... & Müller-Loennies, S. (2009). Analysis of cross-reactive and specific anti-carbohydrate antibodies against lipopolysaccharide from Chlamydophila psittaci. Glycobiology, 20(4), 461-472.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Clone
- S45-18
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- LPS; Lipopolysaccharide; Endotoxins
Related Resources
Rheumatoid Arthritis
Graft-versus-Host Disease
TLR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone S45-18"
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-060 | Recombinant Anti-V. cholerae O1 LPS VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-265 | Recombinant Mouse Anti-LPS Antibody (S25-2) | WB, IHC, FuncS | IgG |
| PABZ-112 | Recombinant Mouse Anti-S. flexneri Lipopolysaccharide Antibody (SYA/J6) | WB, FuncS | IgG |
| FAMAB-0244-CN | Recombinant Chimeric (Mouse/Human) Anti-LPS Antibody (CK3) | WB, ELISA | Chimeric (mouse/human) IgG3, Κ |
| FAMAB-0245-CN | Recombinant Mouse Anti-LPS Antibody (BML-5D11) | ELISA | Mouse IgG2b, λ |
| FAMAB-0246-CN | Recombinant Mouse Anti-LPS Antibody (BML-18F8) | ELISA | Mouse IgG1, λ |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-027 | Recombinant Mouse Anti-C. trachomatis LPS Antibody scFv Fragment (SAG506-01) | WB, ELISA, FuncS | scFv |
| HPAB-J0169-YC-S(P) | Recombinant Human Anti-LPS Antibody scFv Fragment (216-01) | ELISA, FuncS | Human scFv |
| FAMAB-0085WJ-S(P) | Human Anti-LPS Recombinant Antibody (clone H4C5); scFv Fragment | FC, ELISA | Human scFv |
| FAMAB-0086WJ-S(P) | Human Anti-LPS Recombinant Antibody (clone 216); scFv Fragment | FC, ELISA | Human scFv |
| FAMAB-0087WJ-S(P) | Human Anti-LPS Recombinant Antibody (clone HR78); scFv Fragment | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-178 | Recombinant Mouse Anti-F. tularensis LPS Antibody scFv Fragment (Ab52) | WB | scFv |
| FAMAB-0244-CN-F(E) | Recombinant Chimeric (Mouse/Human) Anti-LPS Antibody Fab Fragment (CK3) | WB, ELISA | Chimeric (mouse/human) Fab |
| FAMAB-0245-CN-F(E) | Recombinant Mouse Anti-LPS Antibody Fab Fragment (BML-5D11) | ELISA | Mouse Fab |
| FAMAB-0246-CN-F(E) | Recombinant Mouse Anti-LPS Antibody Fab Fragment (BML-18F8) | ELISA | Mouse Fab |
| FAMAB-0247-CN-F(E) | Recombinant Mouse Anti-LPS Antibody Fab Fragment (BP1-2E7) | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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