Recombinant Humanized Antibody (HuLys11) is capable of binding to Chicken LYZ, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Chicken LYZ mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Chicken LYZ mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Anti-LYZ Recombinant Antibody (PABW-023) in ELISA
ELISA analysis of PABW-023 was performed by coating with chicken egg white lysozyme (0.5 μg/well). Then blocked with BSA and incubated with PABW-023. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-LYZ Recombinant Antibody (PABW-023) in WB
Western blot analysis of PABW-023 was performed with chicken egg white lysozyme in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABW-023 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 3 Anti-LYZ Recombinant Antibody (PABW-023) in DB
Dot Blot analysis of PABW-023 was performed by coating with chicken egg white lysozyme.
PABW-023 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10) | WB, IHC, FuncS | Human IgG |
PABL-268 | Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63) | WB, ELISA, FuncS | Mouse IgG |
PABL-429 | Human Anti-LYZ Recombinant Antibody | WB, IF, FuncS | Human IgG |
FAMAB-0132-CN | Recombinant Humanized Anti-LYZ Antibody (HuD1.3) | ELISA, IF | Humanized IgG2a, Κ |
MRO-0949-CN | Recombinant Rabbit Anti-LYZ Monoclonal Antibody (CBACN-349) | WB, IF, IHC, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10); scFv Fragment | WB, IHC, FuncS | Human scFv |
PSBL-268 | Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
PSBW-128 | Recombinant Mouse Anti-Turkey LYZ Antibody scFv Fragment (1F9) | ELISA | scFv |
PSBC-129 | Recombinant Mouse Anti-Turkey LYZ Antibody scFv Fragment (HyHEL-10 (SFSF) | WB, ELISA | scFv |
PSBS-0138 | Human Anti-LYZ Recombinant Antibody (Clone 01JF38); scFv Fragment | ELISA, FuncS | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-023 | Recombinant Anti-Human LYZ VHH Single Domain Antibody (PNBL-023) | WB, ELISA, FuncS | Llama VHH |
PNBL-024 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody () | WB, ELISA, FuncS | Llama VHH |
PNBL-036 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (PNBL-036) | WB, FuncS | Llama VHH |
PNBL-051 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (PNBL-051) | ELISA | Llama VHH |
PNBL-052 | Recombinant Anti-LYZ (D67H) VHH Single Domain Antibody (PNBL-052) | WB, ELISA, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); Fab Fragment | WB, FuncS | Mouse Fab |
PFBZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1); Fab Fragment | WB, IF, FuncS | Mouse Fab |
PFBW-022 | Human Anti-LYZ Recombinant Antibody (clone dAb HEL4); Fab Fragment | ELISA | Human Fab |
PFBC-023 | Mouse Anti-LYZ Recombinant Antibody (clone F10.6.6); Fab Fragment | WB, IF, FuncS | Mouse Fab |
PFBW-102 | Mouse Anti-LYZ Recombinant Antibody (clone D11.15); Fab Fragment | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
PABX-073-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-138 | Recombinant Human Anti-Lysozyme Antibody | WB, Neut, FuncS | IgG |
PABX-138-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment | WB, Neut, FuncS | scFv |
PABX-139-S (P) | Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) | WB, ELISA, FuncS | Mouse scFv |
PABX-138-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment | WB, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (HyHEL-5) | WB, ELISA, FuncS | scFv |
PABX-074-F (E) | Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (HyHEL-5) | WB, ELISA, FuncS | Fab |
PABX-139-F (E) | Mouse Anti-LYZ Recombinant Antibody; Fab Fragment (PABX-139-F (E)) | WB, ELISA, FuncS | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2112 | Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) | ICC, IHC-P, IP, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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