Recombinant Chimeric (Chicken/Human) Antibody (pT231/pS235_1) is capable of binding to MAPT. The mAb exhibits novel complementarity determining region (CDR) structures with a "bowl-like" conformation in CDR-H2 that tightly and specifically interacts with the phospho-Thr-231 phosphate group, as well as a long, disulfide-constrained CDR-H3 that mediates peptide recognition.
Figure 1 Expression and binding activity of chimeric IgG molecules converted from scFv.
A, Western blot analysis of chimeric IgG molecules converted from scFv clones pT231/pS235_1, pS396/pS404_1, and pT212/pS214_1, respectively. The heavy and light chains for each IgG are indicated by arrows. B–D, ELISA analysis of each purified IgG molecule for binding to the phosphopeptide, non-phosphopeptide, and scrambled (Scr) peptide.
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 2 Surface plasmon resonance kinetic analyses of anti-phospho-tau IgGs for phosphopeptides
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 3 Binding of pathological phospho-tau by antibody pT231/pS235_1 in human AD and Tg4510 transgenic mouse brains.
A–C, immunohistochemical staining of human AD brain (A and B) and healthy brain (C). B, magnification of the boxed area in A. D, Western blot analysis of Tg4510 transgenic and wild-type brain lysates with pT231/pS235_1 IgG (plus control anti-GAPDH antibody). Each lane represents a different animal, and the lysate samples are 3-month-old wild-type mice (lanes 1 and 2), 3-month-old transgenic mice (lanes 3 and 4), 6-month-old wild-type mice (lanes 5 and 6), and 6-month-old transgenic mice (lanes 7 and 8).
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1215z | Mouse Anti-MAPT Recombinant Antibody (clone 44C4) | ICC, IF, IHC, WB | Mouse IgG1 |
PABL-340 | Mouse Anti-MAPT Recombinant Antibody (clone AT8) | ELISA, FuncS | Mouse IgG |
HPAB-0042-WJ | Human Anti-MAPT Recombinant Antibody (HPAB-0042-WJ) | ELISA, WB | Humanized IgG4 |
MRO-1472-CN | Recombinant Rabbit Anti-MAPT Monoclonal Antibody (CBACN-546) | WB, IHC, IP | Rabbit IgG |
MRO-1473-CN | Recombinant Rabbit Anti-MAPT Monoclonal Antibody (CBACN-547) | WB, IF, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-305 | Chicken Anti-MAPT Recombinant Antibody; Fab Fragment | WB, ELISA, FuncS | Chicken Fab |
HPAB-0082-FY-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0082-FY-F(E)) | WB, ELISA | Mouse Fab |
HPAB-0305CQ-F(E) | Mouse Anti-Tau Recombinant Antibody (clone DC-11); Fab Fragment | IF, IHC, WB | Mouse Fab |
HPAB-1104-CN-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-1104-CN-F(E)) | WB, ELISA, IF | Mouse Fab |
HPAB-1105-CN-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-1105-CN-F(E)) | WB, ELISA, IF, Neut | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-674 | Chicken Anti-MAPT Recombinant Antibody (clone pT231/pS235_1); scFv Fragment | WB, ELISA, FACS, Block | Chicken scFv |
NS-090CN-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (NS-090CN-S(P)) | WB, ELISA, IHC, IP | Mouse scFv |
HPAB-0082-FY-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0082-FY-S(P)) | WB, ELISA | Mouse scFv |
HPAB-0042-WJ-S(P) | Human Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0042-WJ-S(P)) | ELISA, WB | Humanized scFv |
HPAB-672-FY-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-672-FY-S(P)) | WB, ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0733MZ | Chicken Anti-TAU Polyclonal IgY | ICC/IF, IHC, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-174 | Recombinant Mouse Anti-Tau5 Antibody | WB, ELISA, Neut, FuncS | IgG |
PABX-174-S (P) | Recombinant Mouse Anti-Tau5 Antibody scFv Fragment | WB, ELISA, Neut, FuncS | scFv |
PABX-174-F (E) | Recombinant Mouse Anti-Tau5 Antibody Fab Fragment | WB, ELISA, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2164 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (DS2164AB) | IHC-P | IgG |
MOR-4513 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH21DS) | WB, IHC-P, FC | IgG |
MOR-4714 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH228DS) | WB, IF, ICC, IHC-P, FC | IgG |
MOR-4715 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH229DS) | WB, IF, ICC, IHC-P | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-090CN | Mouse Anti-MAPT Recombinant Antibody (NS-090CN) | WB, ELISA, IHC, IP, SPR, FuncS | Mouse IgG2b |
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