Mouse Anti-NPC2 Recombinant Antibody (clone 3-6B) (CAT#: HPAB-0225-LSX)
Recombinant monoclonal antibody to NPC2. Clone 3-6B is a mouse IgG2a that can be potentially used in the non-alcoholic steatohepatitis.
Specific Inquiry
WB
Figure 1 Western blot analysis using the mAbs (14-8D, 5-5B, 5-4B, 4-12C, 3-6B, 2-7D, 1-5B and 1-2G) against GST-NPC2 and His-NPC2.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
WB
Figure 2 Western blot analysis using the mAbs against mouse epididymis.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
WB
Figure 3 Epitope region mapping of the monoclonal NPC2 antibodies.
Different length of pNPC2-HA including full-length pNPC2-HA, N-terminal half (1-80 a.a.), C-terminal half (81-151 a.a.) and 41-105 a.a. were transfected into 293T cells and 24 hrs later were harvested for Western blot analysis. Lane 1, 1-2G; Land 2, 1-5B; Lane 3, 2-7B; Lane 4, 3-6B; Lane 5, mAb-HA; Lane 6, 4-12C; Lane 7, 5-4B, Lane 8, 5-5B; Lane 9, 14-8D.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
ELISA
Figure 4 An ELISA plate coated with four peptides covering the 1-40 a.a. region of NPC2 protein was used for epitope mapping.
Representative results from the mAbs (14-8D, 5-4B, 3-6B, 2-7D, 1-5B and 1-2G) are shown.
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
WB
Figure 5 The knockdown effect of shNPC2 on SK-Hep1 cells was detected using anti-NPC2 mAbs (3-6B, 14-8D, 5-4B and 2-7B).
Liao, Y. J., Lin, M. W., Yen, C. H., Lin, Y. T., Wang, C. K., Huang, S. F.,... & Chen, Y. M. A. (2013). Characterization of Niemann-Pick Type C2 protein expression in multiple cancers using a novel NPC2 monoclonal antibody. PLoS One, 8(10).
Specifications
- Immunogen
- GST-NPC2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human NPC2
- Species Reactivity
- Human
- Clone
- 3-6B
- Applications
- ELISA, WB
- Related Disease
- Non-alcoholic steatohepatitis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1717MZ | Recombinant Mouse Anti-Human NPC2 Antibody | ELISA, WB | Mouse antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4348 | Hi-Affi™ Recombinant Rabbit Anti-NPC2 Monoclonal Antibody (SI438DS) | WB, ELISA, IP | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0225-LSX-S(P) | Mouse Anti-NPC2 Recombinant Antibody (clone 3-6B); scFv Fragment | ELISA, WB, EIA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0225-LSX-F(E) | Mouse Anti-NPC2 Recombinant Antibody (clone 3-6B); Fab Fragment | ELISA, WB, EIA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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