Recombinant Mouse Anti-NT5E Antibody (7G2) (CAT#: NEUT-1799CQ)
This antibody is a mouse monoclonal antibody that binds specifically to CD73, and it can neutralize the bioactivity of CD73.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
Figure 2 IHC staining of human endometrium
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
Figure 7 Cervix
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
Specifications
- Immunogen
- Highly purified human placental CD73 protein
- Host Species
- Mouse
- Type
- IgG2a, κ
- Specificity
- Recognizes CD73
- Species Reactivity
- Human
- Clone
- 7G2
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunohistochemistry-Frozen; Immunofluorescence; Immunoprecipitation; Inhibition
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze-thaw cycles.
Target
- Alternative Names
- NT5E; 5-nucleotidase; ecto (CD73); NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase
- Gene ID
- 4907
- UniProt ID
- P21589
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-593MZ | Human Anti-NT5E Recombinant Antibody (TAB-593MZ) | FC | Human IgG |
| TAB-594MZ | Human Anti-NT5E Recombinant Antibody (TAB-594MZ) | FC | Human IgG |
| TAB-595MZ | Human Anti-NT5E Recombinant Antibody (TAB-595MZ) | FC, FuncS | Human IgG |
| TAB-596MZ | Human Anti-NT5E Recombinant Antibody (TAB-596MZ) | FC | Human IgG |
| TAB-441CQ | Anti-Human NT5E Recombinant Antibody (Oleclumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, λ |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1641MZ | Recombinant Mouse Anti-Human NT5E Antibody (clone 20g2) | ICC, WB | Mouse antibody |
| HPAB-N0374-YC | Mouse Anti-NT5E Recombinant Antibody (HPAB-N0374-YC) | ELISA, FC, Inhib | Mouse IgG |
| HPAB-N0375-YC | Human Anti-NT5E Recombinant Antibody (HPAB-N0375-YC) | ELISA, FC, Inhib | Human IgG |
| MRO-0315-CN | Recombinant Rabbit Anti-NT5E Monoclonal Antibody (JM11-40) | WB, IHC | Rabbit IgG |
| HPAB-M0172-YC | Mouse Anti-NT5E Recombinant Antibody (HPAB-M0172-YC) | FuncS | Mouse IgG |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1800CQ | Recombinant Rat Anti-Nt5e Antibody (TY/23) | BL | IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2503 | Hi-Affi™ Recombinant Rabbit Anti-NT5E Monoclonal Antibody (DS2503AB) | WB, IHC, ICC | IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-441CQ | Afuco™ Anti-NT5E ADCC Recombinant Antibody (Oleclumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0172-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-M0172-YC-F(E)) | FC, Inhib | Mouse Fab |
| HPAB-M0173-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-M0173-YC-F(E)) | FC, FuncS | Mouse Fab |
| HPAB-0500-FY-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0500-FY-F(E)) | Neut, FC | Mouse Fab |
| HPAB-0501-FY-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0501-FY-F(E)) | Neut, FC | Mouse Fab |
| HPAB-0502-FY-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0502-FY-F(E)) | Neut, FC | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0196-YC-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0196-YC-S(P)) | FC | Human scFv |
| HPAB-0500-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0500-FY-S(P)) | Neut, FC | Mouse scFv |
| HPAB-0501-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0501-FY-S(P)) | Neut, FC | Mouse scFv |
| HPAB-0502-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0502-FY-S(P)) | Neut, FC | Mouse scFv |
| HPAB-0503-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0503-FY-S(P)) | Neut, FC | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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