Recombinant Mouse Antibody (mAb J16) is capable of binding to PCSK9. This antibody potently inhibits PCSK9 binding to the LDLR extracellular domain and PCSK9-mediated down-regulation of LDLR in vitro.
Figure 1 Human serum titrated with anti-PCSK9 mAb J16.
Raw data trace of fluorescence (in Volts) as a function of time recorded by the KinExA instrument for a typical experiment.
Bee, C., Abdiche, Y. N., Pons, J., & Rajpal, A. (2013). Determining the binding affinity of therapeutic monoclonal antibodies towards their native unpurified antigens in human serum. PLoS One, 8(11), e80501.
Figure 2 PCSK9 follows both direct and indirect internalization routes.
Internalization of J16 bound PCSK9 in liver in negative or APLP2 siRNA treated mice. Scale bars, 10 μM.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 3 ApoB/LDL effects on PCSK9 internalization and function.
Representative western blot showing APLP2, LDLR, ApoB, Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.)
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 4 APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.
Western blot showing APLP2, PCSK9, or Transferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.)
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
Figure 5 ELISA showing LDLR-ECD coated plates.
Shown as average of triplicate samples with SD. J16 effect shown as dotted line.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4), e0125127.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H31 | Human Anti-PCSK9 Recombinant Antibody (TAB-H31) | FC, IP, ELISA, Neut, FuncS, IF, IHC | Human IgG2, λ |
TAB-0953CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0953CL-S(P)) | ELISA | Human scFv |
TAB-0954CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0954CL-S(P)) | WB | Human scFv |
TAB-0955CL-S(P) | Mouse Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0955CL-S(P)) | WB | Mouse scFv |
TAB-0959CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0959CL-S(P)) | ELISA, FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-758 | Anti-PCSK9 Recombinant Antibody (Lodelcizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
TAB-0020CL | Anti-Human PCSK9 Recombinant Antibody (LPD1282/LPD1462 {i.e., antibody 1 F11)) | LDL-c uptake assay | Humanized Antibody |
TAB-0957CL | Anti-Human PCSK9 Recombinant Antibody (h7F8) | ELISA | Humanized antibody |
TAB-0958CL | Anti-Human PCSK9 Recombinant Antibody (h7F8ml2) | ELISA | Humanized antibody |
TAB-0020CL-F(E) | Anti-Human PCSK9 Recombinant Antibody Fab Fragment (LPD1282/LPD1462 {i.e., antibody 1 F11)) | LDL-c uptake assay | Humanized Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-676 | Human Anti-PCSK9 Recombinant Antibody (PABL-676) | WB, FuncS | Human IgG |
PABL-678 | Human Anti-PCSK9 Recombinant Antibody (clone CBL21Q) | ELISA, WB | Human IgG |
HPAB-0810-CN | Human Anti-PCSK9 Recombinant Antibody (HPAB-0810-CN) | ELISA | Human IgG |
HPAB-1439LY | Human Anti-PCSK9 Recombinant Antibody (clone PA4) | WB, ELISA | Human IgG |
HPAB-2454LY | Human Anti-PCSK9 Recombinant Antibody (HPAB-2454LY) | ELISA, Inhib | Chimeric (mouse/human) IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0956CL | Anti-Human PCSK9 Recombinant Antibody (m7F8) | ELISA | |
TAB-0960CL | Anti-Human PCSK9 Recombinant Antibody (Ab1) | ELISA | Rabbit mAb |
TAB-0956CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (m7F8) | ELISA | |
TAB-0960CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (Ab1) | ELISA | Rabbit mAb |
TAB-0956CL-F(E) | Anti-Human PCSK9 Recombinant Antibody Fab Fragment (m7F8) | ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0080CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKE2) | FuncS, Inhib, FC, WB | Single domain antibody |
TAB-0081CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKF8) | FuncS, Inhib, FC, WB | Single domain antibody |
TAB-0082CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKG1) | FuncS, Inhib, FC, WB | Single domain antibody |
TAB-0083CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (P1.70) | FuncS, Inhib, FC, WB | Single domain antibody |
TAB-0084CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (PKE1) | FuncS, Inhib, FC, WB | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1826CQ | Recombinant Human Anti-PCSK9 Antibody, Unconjugated | Neut | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2618 | Hi-Affi™ Recombinant Rabbit Anti-PCSK9 Monoclonal Antibody (DS2618AB) | WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0309-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody (clone 8F5); Fab Fragment | ELISA | Human Fab |
HPAB-0310-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody (clone 3D1); Fab Fragment | ELISA | Human Fab |
HPAB-0311-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody (clone 7c4); Fab Fragment | ELISA | Human Fab |
HPAB-0312-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody (clone 5C2); Fab Fragment | ELISA | Human Fab |
HPAB-0313-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody (clone 4E9); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-758 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Lodelcizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-H10 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Bococizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS | ADCC enhanced antibody |
AFC-TAB-H59 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Ralpancizumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-H31 | Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | Human IgG1, λ |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-M0175-YC-S(P) | Mouse Anti-PCSK9 Recombinant Antibody (clone M/YC-8); scFv Fragment | ELISA | Mouse scFv |
HPAB-0318-YJ-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (HPAB-0318-YJ-S(P)) | Antagonist, ELISA, FuncS | Human scFv |
HPAB-AP781-YC-S(P) | Mouse Anti-PCSK9 Recombinant Antibody; scFv Fragment (HPAB-AP781-YC-S(P)) | ELISA, WB | Mouse scFv |
HPAB-AP782-YC-S(P) | Mouse Anti-PCSK9 Recombinant Antibody; scFv Fragment (HPAB-AP782-YC-S(P)) | ELISA, WB | Mouse scFv |
HPAB-AP783-YC-S(P) | Mouse Anti-PCSK9 Recombinant Antibody; scFv Fragment (HPAB-AP783-YC-S(P)) | ELISA, WB | Mouse scFv |
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