Anti-Human PDGFD Recombinant Antibody (CR002) (CAT#: TAB-0987CL)

Figure 1 PDGF-D stimulates the proliferation of rat fibroblasts in vitro .

Incorporation of BrdU in growth-arrested rat fibroblasts NRK-49F after stimulation with PDGF-BB and -DD (10 and100 ng/ml, respectively) with or without the anti-PDGF-D fully human monoclonal antibody CR002 (1 μg/ml).

Boor, P., Konieczny, A., Villa, L., Kunter, U., van Roeyen, C. R., LaRochelle, W. J., ... & Floege, J. (2007). PDGF-D inhibition by CR002 ameliorates tubulointerstitial fibrosis following experimental glomerulonephritis. Nephrology Dialysis Transplantation, 22(5), 1323-1331.

Figure 2 Development of proteinuria was transiently decreased by CR002.

Proteinuria was significantly ameliorated by the treatment at days 49, 56 and 77. Arrows indicate the time points of CR002 administration. The insert shows the concentration of circulating CR002 on days 42, 49 and 56 and the grey area represents the approximate duration of effective PDGF-D inhibition.

Boor, P., Konieczny, A., Villa, L., Kunter, U., van Roeyen, C. R., LaRochelle, W. J., ... & Floege, J. (2007). PDGF-D inhibition by CR002 ameliorates tubulointerstitial fibrosis following experimental glomerulonephritis. Nephrology Dialysis Transplantation, 22(5), 1323-1331.

Figure 3 Sirius red staining ( A , B ) and specific immunohistochemistry for collagen type I ( C , D) and III ( E , F ), vimentin ( G , H ) and α-SMA ( I , J ) in IgG-treated control animals (images A, C, E, G, I) or CR002-treated animals (B, D, F, H, J).

Boor, P., Konieczny, A., Villa, L., Kunter, U., van Roeyen, C. R., LaRochelle, W. J., ... & Floege, J. (2007). PDGF-D inhibition by CR002 ameliorates tubulointerstitial fibrosis following experimental glomerulonephritis. Nephrology Dialysis Transplantation, 22(5), 1323-1331.

Figure 4 Renal interstitial fibrosis as detected by Sirius red staining.

Morphometric evaluation of Sirius red–positive area in the renal cortex.

Ostendorf, T., Rong, S., Boor, P., Wiedemann, S., Kunter, U., Haubold, U., ... & Alvarez, E. (2006). Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis. Journal of the American Society of Nephrology, 17(4), 1054-1062.

Figure 5 Expression of desmin at the glomerular edge on day 56 after disease induction.

Ostendorf, T., Rong, S., Boor, P., Wiedemann, S., Kunter, U., Haubold, U., ... & Alvarez, E. (2006). Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis. Journal of the American Society of Nephrology, 17(4), 1054-1062.

Figure 6 Renal cortical deposition of type III collagen.

Morphometric evaluation of the area that stained positively for type III collagen in the renal cortex.

Ostendorf, T., Rong, S., Boor, P., Wiedemann, S., Kunter, U., Haubold, U., ... & Alvarez, E. (2006). Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis. Journal of the American Society of Nephrology, 17(4), 1054-1062.

Figure 7 Renal cortical deposition of type III collagen.

Morphometric evaluation of the area that stained positively for fibronectin in the renal cortex.

Ostendorf, T., Rong, S., Boor, P., Wiedemann, S., Kunter, U., Haubold, U., ... & Alvarez, E. (2006). Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis. Journal of the American Society of Nephrology, 17(4), 1054-1062.

Figure 8 Renal interstitial detection of membrane attack complex (C5b-9).

Morphometric evaluation of C5b-9 –positive area in the renal cortex.

Ostendorf, T., Rong, S., Boor, P., Wiedemann, S., Kunter, U., Haubold, U., ... & Alvarez, E. (2006). Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis. Journal of the American Society of Nephrology, 17(4), 1054-1062.