Afuco™ Anti-Human SLC2A8 ADCC Recombinant Antibody (VB1-050), ADCC Enhanced (CAT#: AFC-551CL)

Figure 1 demonstrates antibody cell surface binding after incubation of A-375 cells at different temperatures as determined by flow cytometry.

Fluorescence labeling of A-375 cells after incubation of cell suspensions at 4°C: 4B5 (1) and VB1-050 (2). Fluorescence labeling of A-375 cells after warming antibody-bound cells to 37°C: VB1-050 for 60 min (3), for 120 min (4).

Figure 2 shows confocal microscopy assessment of VB1-050 internalization.

A-375 cells were incubated with antibody at 4°C, washed and warmed to 37°C for 60 min. Cells were fixed, permeabilized and labeled with fluorescent-labeled second antibody. Fluorescence labeling of A-375 cells after incubation of VB1-050 at 4°C for 60 min, displaying circumferential surface distribution of labeling, magnification (A). Following incubation of antibody-bound cells at 37°C for 60 min the cells show strong intracellular staining by internalized antibody, magnification (B).

Figure 3 shows the in vitro cytotoxicity of VB6-050.

MTS assay of VB6-050 with antigen-positive cells MB-435S (open circle) and antigen-negative cells Daudi (black circle). Cells seeded at 1000 cells per well, were incubated with the Fab-de-bouganin purified proteins. After 5 days incubation, the cell viability was measured and IC50 was determined.